論文

査読有り
2016年7月

The Parathyroid Hormone Second Receptor PTH2R and its Ligand Tuberoinfundibular Peptide of 39 Residues TIP39 Regulate Intracellular Calcium and Influence Keratinocyte Differentiation

JOURNAL OF INVESTIGATIVE DERMATOLOGY
  • Emi Sato
  • ,
  • Jun Muto
  • ,
  • Ling-Juan Zhang
  • ,
  • Christopher A. Adase
  • ,
  • James A. Sanford
  • ,
  • Toshiya Takahashi
  • ,
  • Teruaki Nakatsuji
  • ,
  • Ted B. Usdin
  • ,
  • Richard L. Gallo

136
7
開始ページ
1449
終了ページ
1459
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jid.2016.02.814
出版者・発行元
ELSEVIER SCIENCE INC

Genes related to the parathyroid hormone (PTH) influence cutaneous immune defense and development, but the full functions of the PTH family in cutaneous biology remain incompletely understood. In this study, we examined the expression and potential functions of the PTH second receptor (PTH2R) and its ligand, the tuberoinfundibular peptide of 39 residues (TIP39), in the skin. TIP39 and PTH2R mRNA and protein were detectable in both human and mouse skin, and in cultured keratinocytes and adipocytes. TIP39 was observed in the basal layer of human skin, whereas PTH2R was detected in the spinous to granular layer. The subcellular localization of TIP39 in keratinocytes changed during calcium-induced differentiation and shifted to colocalize with PTH2R at the membrane. The addition of recombinant TIP39 to normal human keratinocytes in culture induced an increase in intercellular calcium and triggered aspects of terminal differentiation including decreased keratin-14 and increased involucrin expression. Consistent with these observations, PTH2R(-/-) ice were observed to have increased epidermal thickness. In summary, identification of TIP39 and its receptor in the epidermis reveals an additional PTH family member that is expressed in the skin and may influence keratinocyte function.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1016/j.jid.2016.02.814
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27000502
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000379016400022&DestApp=WOS_CPL

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