論文

査読有り
2013年6月

Vitamin D-dependent cathelicidin inhibits Mycobacterium marinum infection in human monocytic cells

JOURNAL OF DERMATOLOGICAL SCIENCE
  • Emi Sato
  • ,
  • Shinichi Imafuku
  • ,
  • Kazunari Ishii
  • ,
  • Ryota Itoh
  • ,
  • Bin Chou
  • ,
  • Toshinori Soejima
  • ,
  • Juichiro Nakayama
  • ,
  • Kenji Hiromatsu

70
3
開始ページ
166
終了ページ
172
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jdermsci.2013.01.011
出版者・発行元
ELSEVIER IRELAND LTD

Background: 1 alpha,25-Dihydroxyvitamin D3 (1,25(OH)2D3) up-regulates the production of human cathelicidin antimicrobial peptide (CAMP) from monocytes/macrophages infected with Mycobacterium tuberculosis (M. tbc). CAMP facilitates the co-localization of autophagolysosomes with M. tbc, promoting the antimicrobial activity of monocytes. Mycobacterium marinum (M. marinum) is an acid-fast bacillus that causes less severe granulomatous skin lesions compared with M. tbc.
Objective: We investigated whether autophagic antimicrobial activity is promoted by 1,25(OH)2D3 or C-terminal of cathelicidin LL-37 in human monocytes upon infection with M. marinum.
Methods: Human monocytes (THP-1) were infected with M. marinum. Effects of simultaneous treatments of 1,25(OH)2D3, exogenous LL-37 peptide, autophagolysosome inhibitors, 3-methyladenine or chloroquine, were examined.
Results: CAMP was strongly induced by adding 1,25(OH)2D3 to the culture of THP-1 cells. In the absence of 1,25(OH)2D3 M. marinum infection alone did not induce CAMP, however, simultaneous addition of 1,25(OH)2D3 to M. marinum infection accelerated CAMP production more than 1,25(OH)2D3 alone. Proliferation of M. marinum was markedly decreased in the presence of 1,25(OH)2D3 or exogenous LL-37 in THP-1 cells. Co-localization of CAMP with autophagolysosome was evident in 1,25(OH)2D3 and LL-37 treated THP-1 cells after M. marinum infection. Autophagolysosome inhibitors abrogated the antimicrobial effects of 1,25(OH)2D3 and exogenous LL-37 against M. marinum infection in THP-1 cells.
Conclusions: Human monocytic cells, whose CAMP production is up-regulated by 1,25(OH)2D3-vitamin D receptor pathway, accelerate antimicrobial function of autophagolysosome in M. marinum infection. (c) 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

Web of Science ® 被引用回数 : 29

リンク情報
DOI
https://doi.org/10.1016/j.jdermsci.2013.01.011
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23452544
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000320146200003&DestApp=WOS_CPL

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