2015年3月
New enzymatic methods for selective assay of L-lysine using an L-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395
Journal of Bioscience and Bioengineering
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- ,
- ,
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- 巻
- 119
- 号
- 3
- 開始ページ
- 369
- 終了ページ
- 374
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jbiosc.2014.08.013
- 出版者・発行元
- Elsevier {BV}
We developed new enzymatic methods for the selective assay of L-lysine by utilizing an oxidase reaction and a decarboxylation reaction by the L-lysine-specific decarboxylase/oxidase (L-Lys-DC/OD) from Burkholderia sp. AIU 395. The method utilizing the oxidase reaction of this enzyme was useful for determination of high concentrations of L-lysine. The method utilizing the decarboxylase reaction, which proceeded via the combination of the L-Lys-DC/OD and putrescine oxidase (PUO) from Micrococcus rubens, was effective for determination of low concentrations of L-lysine. Both methods showed good linearity, and neither was affected by other amino acids or amines. In addition, the within-assay and between-assay precisions of both methods were within the allowable range. The coupling of L-Lys-DC/OD with PUO was also useful for the differential assay of L-lysine and cadaverine. These newly developed methods were applied to the assay of L-lysine in biological samples and found to be effective.
- リンク情報
- ID情報
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- DOI : 10.1016/j.jbiosc.2014.08.013
- ISSN : 1389-1723
- eISSN : 1347-4421
- ORCIDのPut Code : 21559927
- PubMed ID : 25282636
- Web of Science ID : WOS:000351808700020