Eiji Ohashi - My portal - researchmap

Profile Information

Affiliation: Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology

Degree: Doctor of Science(Mar, 2002, Kyoto University)

Researcher number: 90378951
ORCID ID: https://orcid.org/0000-0002-1771-5585
J-GLOBAL ID: 201801007639591185
researchmap Member ID: B000331397

2024年2月20日にプレスリリース配信を行いました。

https://www.nagahama-i-bio.ac.jp/?p=50007

Research Areas

1

Life sciences / Molecular biology /

Research Interests

4

Education

3

Apr, 1999 - Mar, 2002

理学研究科 (生物科学専攻) 博士後期課程, 京都大学大学院
Apr, 1997 - Mar, 1999

理学研究科 (生物科学専攻) 修士課程, 京都大学大学院
Apr, 1993 - Mar, 1997

Faculty of Science, Kyoto University

Research History

8

Apr, 2023 - Present

Associate professor, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology
Oct, 2021 - Mar, 2023

Lecturer, Faculty of Science, Department of Biology, Kyushu University
Oct, 2008 - Sep, 2021

Assistant professor, Faculty of Science, Department of Biology, Kyushu University
Mar, 2007 - Oct, 2008

Visiting Fellow, NICHD, National Institutes of Health
Aug, 2006 - Feb, 2007

Assistant professor, Institute of Virus Research, Kyoto University
Apr, 2003 - Jul, 2006

研究員（科学研究）, 京都大学ウイルス研究所（現・医生物学研究所）
Apr, 2002 - Mar, 2003

日本学術振興会特別研究員（PD）
Apr, 2001 - Mar, 2002

日本学術振興会特別研究員（DC2）

Papers

32

Resection of DNA double-strand breaks activates Mre11-Rad50-Nbs1- and Rad9-Hus1-Rad1-dependent mechanisms that redundantly promote ATR checkpoint activation and end processing in Xenopus egg extracts.

Kensuke Tatsukawa, Reihi Sakamoto, Yoshitaka Kawasoe, Yumiko Kubota, Toshiki Tsurimoto, Tatsuro S Takahashi, Eiji Ohashi

Nucleic acids research, Feb 13, 2024 Peer-reviewed
Structural basis for intra- and inter-molecular interactions on RAD9 subunit of 9-1-1 checkpoint clamp implies functional 9-1-1 regulation by RHINO.

Kodai Hara, Kensuke Tatsukawa, Kiho Nagata, Nao Iida, Asami Hishiki, Eiji Ohashi, Hiroshi Hashimoto

The Journal of biological chemistry, 105751-105751, Feb 12, 2024 Peer-reviewed
The 9-1-1 DNA clamp subunit RAD1 forms specific interactions with clamp loader RAD17, revealing functional implications for binding-protein RHINO

Kodai Hara, Asami Hishiki, Takako Hoshino, Kiho Nagata, Nao Iida, Yukimasa Sawada, Eiji Ohashi, Hiroshi Hashimoto

Journal of Biological Chemistry, 299(4) 103061-103061, Feb 24, 2023 Peer-reviewed
Structure of the RAD9-RAD1-HUS1 checkpoint clamp bound to RHINO sheds light on the other side of the DNA clamp

Kodai Hara, Nao Iida, Ryota Tamafune, Eiji Ohashi, Hitomi Sakurai, Yoshinobu Ishikawa, Asami Hishiki, Hiroshi Hashimoto

Journal of Biological Chemistry, 295(4) 899-904, Jan 24, 2020 Peer-reviewed
Specific basic patch-dependent multimerization of Saccharomyces cerevisiae ORC on single-stranded DNA promotes ATP hydrolysis

Hironori Kawakami, Ryuya Muraoka, Eiji Ohashi, Kenta Kawabata, Shota Kanamoto, Takeaki Chichibu, Toshiki Tsurimoto, Tsutomu Katayama

Genes to Cells, 24(9) 608-618, Sep 1, 2019 Peer-reviewed
Human CTF18-RFC clamp-loader complexed with non-synthesising DNA polymerase ϵ efficiently loads the PCNA sliding clamp

Ryo Fujisawa, Eiji Ohashi, Kouji Hirota, Toshiki Tsurimoto

Nucleic Acids Research, 45(8) 4550-4563, May 5, 2017 Peer-reviewed
Multiple regulatory roles of Rad9 C-tail in DNA damage responses

Eiji Ohashi

Journal of Rare Diseases Research & Treatment, 2(3) 16-21, Apr, 2017 Peer-reviewed
Conserved interaction of Ctf18-RFC with DNA polymerase ε is critical for maintenance of genome stability in Saccharomyces cerevisiae

Hiroko Okimoto, Seiji Tanaka, Hiroyuki Araki, Eiji Ohashi, Toshiki Tsurimoto

Genes to Cells, 21(5) 482-491, May 1, 2016 Peer-reviewed
Rapid purification and characterization of mutant origin recognition complexes in Saccharomyces cerevisiae

Hironori Kawakami, Eiji Ohashi, Toshiki Tsurimoto, Tsutomu Katayama

Frontiers in Microbiology, 7(APR) 521-521, Apr, 2016 Peer-reviewed
Specific binding of eukaryotic ORC to DNA replication origins depends on highly conserved basic residues

Hironori Kawakami, Eiji Ohashi, Shota Kanamoto, Toshiki Tsurimoto, Tsutomu Katayama

Scientific Reports, 5 14929-14929, Oct 12, 2015 Peer-reviewed
Intramolecular binding of the Rad9 C terminus in the checkpoint clamp Rad9-Hus1-Rad1 is closely linked with its DNA binding

Yukimasa Takeishi, Rie Iwaya-Omi, Eiji Ohashi, Toshiki Tsurimoto

Journal of Biological Chemistry, 290(32) 19923-19932, Aug 7, 2015 Peer-reviewed
Interaction between Rad9-Hus1-Rad1 and TopBP1 activates ATR-ATRIP and promotes TopBP1 recruitment to sites of UV-damage

Eiji Ohashi, Yukimasa Takeishi, Satoshi Ueda, Toshiki Tsurimoto

DNA Repair, 21 1-11, Sep 1, 2014 Peer-reviewed
Ubiquitin mediates the physical and functional interaction between human DNA polymerases η and ι

Justyna McIntyre, Antonio E. Vidal, Mary P. McLenigan, Martha G. Bomar, Elena Curti, John P. McDonald, Brian S. Plosky, Eiji Ohashi, Roger Woodgate

Nucleic Acids Research, 41(3) 1649-1660, Feb, 2013 Peer-reviewed
Two serine phosphorylation sites in the C-terminus of Rad9 are critical for 9-1-1 binding to TopBP1 and activation of the DNA damage checkpoint response in HeLa cells

Satoshi Ueda, Yukimasa Takeishi, Eiji Ohashi, Toshiki Tsurimoto

Genes to Cells, 17(10) 807-816, Oct, 2012 Peer-reviewed
The vital role of polymerase ζ and REV1 in mutagenic, but not correct, DNA synthesis across Benzo[a]pyrene-dG and recruitment of polymerase ζ by REV1 to replication-stalled site

Keiji Hashimoto, Youngjin Cho, In Young Yang, Jun Ichi Akagi, Eiji Ohashi, Satoshi Tateishi, Niels De Wind, Fumio Hanaoka, Haruo Ohmori, Masaaki Moriya

Journal of Biological Chemistry, 287(12) 9613-9622, Mar 16, 2012 Peer-reviewed
Human replicative DNA polymerase δ can bypass T-T (6-4) ultraviolet photoproducts on template strands

Takeo Narita, Toshiki Tsurimoto, Junpei Yamamoto, Kana Nishihara, Kaori Ogawa, Eiji Ohashi, Terry Evans, Shigenori Iwai, Shunichi Takeda, Kouji Hirota

Genes to Cells, 15(12) 1228-1239, Dec, 2010 Peer-reviewed
Stable interaction between the human proliferating cell nuclear antigen loader complex Ctf18-Replication Factor C (RFC) and DNA polymerase ε is mediated by the cohesion-specific subunits, Ctf18, Dcc1, and Ctf8

Takeshi Murakami, Ryuji Takano, Satoshi Takeo, Rina Taniguchi, Kaori Ogawa, Eiji Ohashi, Toshiki Tsurimoto

Journal of Biological Chemistry, 285(45) 34608-34615, Nov 5, 2010 Peer-reviewed
Casein kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1

Yukimasa Takeishi, Eiji Ohashi, Kaori Ogawa, Hisao Masai, Chikashi Obuse, Toshiki Tsurimoto

Genes to Cells, 15(7) 761-771, Jun, 2010 Peer-reviewed
Overlapping in short motif sequences for binding to human REV7 and MAD2 proteins

Tomo Hanafusa, Toshiyuki Habu, Junya Tomida, Eiji Ohashi, Yoshiki Murakumo, Haruo Ohmori

Genes to Cells, 15(3) 281-296, Mar, 2010 Peer-reviewed
Interaction with DNA polymerase η is required for nuclear accumulation of REV1 and suppression of spontaneous mutations in human cells

Jun-ichi Akagi, Chikahide Masutani, Yuki Kataoka, Takashi Kan, Eiji Ohashi, Toshio Mori, Haruo Ohmori, Fumio Hanaoka

DNA Repair, 8(5) 585-599, May 1, 2009 Peer-reviewed

More

Misc.

6

Unique and shared functions of the Rad9–Hus1–Rad1 and Mre11–Rad50–Nbs1 complexes in ATR checkpoint activation and long-range DNA end resection in Xenopus egg extracts

Kensuke Tatsukawa, Reihi Sakamoto, Yoshitaka Kawasoe, Yumiko Kubota, Toshiki Tsurimoto, Tatsuro S. Takahashi, Eiji Ohashi

bioRxiv, Aug 10, 2023
Mechanisms how ORC recognizes chromosomal replication origins in budding yeast

Hironori Kawakami, Tomohito Kawanishi, Eiji Ohashi, Shota Kanamoto, Toshiki Tsurimoto, Tsutomu Katayama

Genes & Genetic Systems, 90(6) 367, Dec, 2015
Regulation of ORC: structure-function relationship of Orc1-Orc6 interaction

Hironori Kawakami, Eiji Ohashi, Tomohito Kawanishi, ToshikiTsurimoto, Tsutomu Katayama

Genes & Genetic Systems, 89(6) 323, Dec, 2014
Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa

Eiji Ohashi, Tomoo Ogi, Rika Kusumoto, Shigenori Iwai, Chikahide Masutani, Fumio Hanaoka, Haruo Ohmori

Genes & Genetic Systems, 75(6) 387, Dec, 2000
Studies on the Escherichia coli dinB gene function

Eiji Ohashi, Ken Hayashi, Haruo Ohmori

Genes & Genetic Systems, 73(6) 419, Dec, 1998
Studies on the Escherichia coli dinB/P gene function

Eiji Ohashi, Ken Hayashi, Haruo Ohmori

Genes & Genetic Systems, 72(6) 406, Dec, 1997

Books and Other Publications

9

Advances in Experimental Medicine and Biology “DNA Replication from Old Principles to New Discoveries”

Eiji Ohashi, Toshiki Tsurimoto (Role: Contributor, Vol.1042, pp.135-162: Functions of Multiple Clamp and Clamp-Loader Complexes in Eukaryotic DNA Replication)

Springer, Jan, 2018
Advances in Protein Chemistry & Structural biology

Haruo Ohmori, Tomo Hanafusa, Eiji Ohashi, Cyrus Vaziri (Role: Contributor)

Nov 27, 2009
New Developments in Mutation Research

Manabu Kawanishi, Kazuki Matsukawa, Eiji Ohashi, Takeji Takamura, Yukari Totsuka, Masahiko Watanabe, Takashi Sugimura, Keiji Wakabayashi, Fumio Hanaoka, Haruo Ohmori, Takashi Yagi (Role: Contributor, Chapter 6, pp.133-148: Translesion DNA synthesis across mono ADP-ribosylated dG by Y-family DNA polymerases)

Nova Science Publishers, Feb 28, 2007
Advances in Protein Chemistry “DNA Repair and Replication”

Haruo Ohmori, Eiji Ohashi, Tomoo Ogi (Role: Contributor)

Dec 13, 2004
医学のあゆみ「発がん過程における染色体ダイナミクス」

大橋英治, 大森治夫 (Role: Contributor, 208巻10号, 849-852頁: 損傷乗り越えDNA合成と発がん)

医歯薬出版, Mar 6, 2004
生化学「真核生物のDNAポリメラーゼ・スーパーファミリー」

大橋英治, 荻朋男, 大森治夫 (Role: Contributor, 74巻3号, 218-223頁: ほ乳類DNAポリメラーゼκの生化学的性質とその発現制御.)

日本生化学会, Mar 25, 2002
蛋白質核酸酵素「DNA修復ネットワークとその破綻の分子機構」

荻朋男, 大橋英治, 大森治夫 (Role: Contributor, 46巻8号, 1155-1161頁: 大腸菌DinBとそのほ乳類ホモログPolκによる突然変異誘発機構)

共立出版, Jun 10, 2001
細胞工学「DNA修復機構 – DNA複製、チェックポイントとのクロストーク」

大橋英治, 大森治夫 (Role: Contributor, 19巻10号, 1448-1453頁: 大腸菌におけるDNA複製とDNA修復のクロストーク)

秀潤社, Sep 22, 2000
医学のあゆみ「DNA修復機構の医学的意義の新展開」

大橋英治, 大森治夫 (Role: Contributor, 194巻9号, 684-685頁: 大腸菌dinB遺伝子とヒトホモログの機能)

医歯薬出版, Aug 26, 2000

Presentations

50

DNA損傷チェックポイントクランプ9-1-1のリング部分の役割

達川絢介, 橋本博, 高橋達郎, 大橋英治

第44回日本分子生物学会年会横浜, Dec 1, 2021
DNA二重鎖切断後の削り込みとチェックポイント活性化の連携機構

達川絢介, 高橋達郎, 大橋英治

第26回DNA複製・組換え・修復ワークショップオンライン, Oct 23, 2021
DNA損傷チェックポイントクランプ9-1-1によるATR活性化機構

達川絢介, 橋本博, 釣本敏樹, 高橋達郎, 大橋英治

第38回染色体ワークショップ第19回核ダイナミクス研究会オンライン, Jan 18, 2021
チェックポイントセンサー9-1-1とMRNによる二重鎖切断後の損傷応答制御

達川絢介, 釣本敏樹, 高橋達郎, 大橋英治

第43回日本分子生物学会年会オンライン, Dec 4, 2020
Rad9 C-terminal tail intramolecularly binds to the Rad9-Hus1-Rad1 checkpoint clamp and regulates the binding of DNA damage response proteins

Eiji Ohashi, Naoya Iwata, Kensuke Tatsukawa, Toshiki Tsurimoto

The 11th International 3R & 3C (Replication, Recombination, and Repair & Cell Cycle, Chromosome, and Chromatin) Symposium 金沢, Nov 13, 2018
Rad9-Hus1-Rad1クランプのRad9 C-tailを介したDNA複製ストレス応答の制御機構

大橋英治, 岩田直也, 達川絢介, 釣本敏樹

第40回日本分子生物学会年会神戸, Dec 6, 2017 Invited
9-1-1チェックポイントクランプの分子内結合によるp21と9-1-1の結合制御

大橋英治, 岩田直也, 達川絢介, 釣本敏樹

第24回DNA複製・組換え・修復ワークショップ岐阜, Nov 28, 2017
Intramolecular binding of Rad9 C-terminal tail to Rad9-Hus1-Rad1 core ring structure regulates the binding of DNA damage response proteins

Eiji Ohashi, Naoya Iwata, Kensuke Tatsukawa, Toshiki Tsurimoto

Cold Spring Harbor Laboratory Meeting on “Eukaryotic DNA replication and genome maintenance” Cold Spring Harbor, NY, USA, Sep 7, 2017
Rad9 C末端による9-1-1クランプへの蛋白質結合の制御

大橋英治, 岩田直也, 釣本敏樹

第34回染色体ワークショップ・第15回核ダイナミクス研究会木更津, Jan 11, 2017
PCNA結合蛋白質のRad9-Hus1-Rad1リングとの結合メカニズム

大橋英治, 岩田直也, 釣本敏樹

第39回日本分子生物学会年会横浜, Dec 2, 2016 Invited
Intramolecular folding of Rad9 C-terminal tail in the Rad9-Hus1-Rad1 checkpoint clamp regulates its interaction with DNA damage response proteins

Eiji Ohashi, Naoya Iwata, Toshiki Tsurimoto

The 10th International 3R (Replication, Recombination and Repair) Symposium 松江, Nov 15, 2016
Rad9 C末端によるチェックポイントクランプRad9-Hus1-Rad1の機能制御機構

大橋英治, 武石幸容, 釣本敏樹

第38回日本分子生物学会年会神戸, Dec 3, 2015 Invited
Rad9 C末端によるRad9-Hus1-Rad1(9-1-1)のDNA損傷応答制御機構

大橋英治, 武石幸容, 釣本敏樹

第23回DNA複製・組換え・修復ワークショップ焼津, Oct 19, 2015
Multiple roles of Rad9 C-tail in the checkpoint clamp Rad9-Hus1-Rad1

Eiji Ohashi, Yukimasa Takeishi, Toshiki Tsurimoto

Cold Spring Harbor Laboratory Meeting on “Eukaryotic DNA replication and genome maintenance” Cold Spring Harbor, NY, USA, Sep 3, 2015
DNA損傷応答因子ATR-ATRIP, Rad9-Hus1-Rad1, TopBP1の3者間結合によるATR活性化機構

大橋英治、武石幸容、釣本敏樹

第37回日本分子生物学会年会横浜, Nov 27, 2014 Invited
The ATR activation through the accumulation of the checkpoint factors and their interactions at the site of DNA damage

Eiji Ohashi, Yukimasa Takeishi, Toshiki Tsurimoto

The 9th International 3R (Replication, Recombination and Repair) Symposium 御殿場, Nov 20, 2014
ヒトTopBP1-Rad9結合によるATR活性化を介したTopBP1のDNA損傷部位への局在促進

大橋英治, 武石幸容, 上田聡, 釣本敏樹

がん研究分野の特性等を踏まえた支援活動公開シンポジウム東京, Jan 31, 2014
Rad9-TopBP1間の結合はATRの活性化を介してTopBP1のDNA損傷部位への局在を促進する

大橋英治, 武石幸容, 上田聡, 釣本敏樹

第36回日本分子生物学会年会神戸, Dec 3, 2013 Invited
ヒトTopBP1-Rad9結合によるATR活性化を介したTopBP1のDNA損傷部位への局在促進

大橋英治, 武石幸容, 上田聡, 釣本敏樹

第22回DNA複製・組換え・修復ワークショップ仙台, Nov 20, 2013
The positive feedback cycle of ATR-activation and TopBP1 accumulation triggered by interaction of Rad9-Hus1-Rad1 with TopBP1

Eiji Ohashi, Yukimasa Takeishi, Satoshi Ueda, Toshiki Tsurimoto

Cold Spring Harbor Laboratory Meeting on “Eukaryotic DNA replication and genome maintenance” Cold Spring Harbor, NY, USA, Sep 11, 2013

More

Teaching Experience

12

2023 - Present

エッセンシャル生物学I (Nagahama Institute of Bio-Science and Technology)
2023 - Present

応用実験I, II (Nagahama Institute of Bio-Science and Technology)
2023 - Present

自然科学基礎実験I, II, III (Nagahama Institute of Bio-Science and Technology)
2009 - 2023

生物学演習I (Kyushu University)
2009 - 2023

自然科学総合実験 (Kyushu University)
2021 - 2022

基礎遺伝学実験 (九州大学)
2022

生物科学特論Ⅱ (九州大学大学院)
2022

生物科学I (九州大学大学院)
2022

Biophysics (Kyushu University)
2013 - 2020

応用生物化学実験 (Kyushu University)
2008 - 2011

染色体機能学実験 (Kyushu University)
2009

総合演習「環境と生物」 (Kyushu University)

Professional Memberships

2

2000 - Present

日本分子生物学会
1997 - 2000

日本遺伝学会

Research Projects

7

DNA損傷応答クランプ9-1-1の装着から脱装着までの反応ネットワークの解析

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2019 - Mar, 2022

大橋英治
DNA損傷応答キナーゼATR活性化の分子機構

豊田理研スカラー, 豊田理化学研究所, Apr, 2020 - Mar, 2021
Studies on PCNA loading onto newly synthesized leading DNA by Ctf18-RFC/Pole complex

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2016 - Mar, 2019

Tsurimoto Toshiki
Analysis of the early steps of DNA damage response through 9-1-1/TopBP1 interaction

Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Grant-in-Aid for Young Scientists (B), Japan Society for the Promotion of Science, Apr, 2010 - Mar, 2012

OHASHI Eiji, TSURIMOTO Toshiki
ヒト細胞DNA損傷応答系の試験管内再構築

研究奨励金, 上原記念生命科学財団, Apr, 2010 - Mar, 2011
損傷乗り越え複製酵素複合体のダイナミクス

科学研究費助成事業, 若手研究(B), 日本学術振興会, Apr, 2005 - Mar, 2007

大橋英治
ヒトDNAポリメラーゼκによる突然変異誘発機構の解明

科学研究費助成事業, 特別研究員奨励費, 日本学術振興会, Apr, 2001 - Mar, 2003

大橋英治

Academic Activities

11

第38回染色体ワークショップ・第19回核ダイナミクス研究会

Panel moderator, session chair, etc.

Jan 18, 2021
第40回日本分子生物学会年会

Panel moderator, session chair, etc.

Dec 6, 2017
第24回DNA複製・組換え・修復ワークショップ

Panel moderator, session chair, etc.

Nov 28, 2017
第39回日本分子生物学会年会

Panel moderator, session chair, etc.

Dec 2, 2016
第23回DNA複製・組換え・修復ワークショップ

Panel moderator, session chair, etc.

Oct 19, 2015
第37回日本分子生物学会年会

Panel moderator, session chair, etc.

Nov 25, 2014
第36回日本分子生物学会年会

Panel moderator, session chair, etc.

Dec 3, 2013
第22回DNA複製・組換え・修復ワークショップ

Panel moderator, session chair, etc.

Nov 20, 2013
第34回日本分子生物学会年会

Panel moderator, session chair, etc.

Dec 13, 2011
第21回DNA複製・組換え・ゲノム安定性制御ワークショップ

Panel moderator, session chair, etc.

Oct 25, 2011
International Conference on Radiation and Cancer Biology 2010 at Nagasaki

Planning, management, etc., Panel moderator, session chair, etc.

Feb 17, 2010 - Feb 20, 2010