2020年6月26日
Epigenetic regulation of spurious transcription initiation in Arabidopsis.
Nature communications
- ,
- ,
- ,
- ,
- ,
- 巻
- 11
- 号
- 1
- 開始ページ
- 3224
- 終了ページ
- 3224
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41467-020-16951-w
In plants, epigenetic regulation is critical for silencing transposons and maintaining proper gene expression. However, its impact on the genome-wide transcription initiation landscape remains elusive. By conducting a genome-wide analysis of transcription start sites (TSSs) using cap analysis of gene expression (CAGE) sequencing, we show that thousands of TSSs are exclusively activated in various epigenetic mutants of Arabidopsis thaliana and referred to as cryptic TSSs. Many have not been identified in previous studies, of which up to 65% are contributed by transposons. They possess similar genetic features to regular TSSs and their activation is strongly associated with the ectopic recruitment of RNAPII machinery. The activation of cryptic TSSs significantly alters transcription of nearby TSSs, including those of genes important for development and stress responses. Our study, therefore, sheds light on the role of epigenetic regulation in maintaining proper gene functions in plants by suppressing transcription from cryptic TSSs.
- リンク情報
- ID情報
-
- DOI : 10.1038/s41467-020-16951-w
- PubMed ID : 32591528
- PubMed Central 記事ID : PMC7319988