論文

査読有り 国際誌
2020年3月19日

Liquidity Is a Critical Determinant for Selective Autophagy of Protein Condensates.

Molecular cell
  • Akinori Yamasaki
  • ,
  • Jahangir Md Alam
  • ,
  • Daisuke Noshiro
  • ,
  • Eri Hirata
  • ,
  • Yuko Fujioka
  • ,
  • Kuninori Suzuki
  • ,
  • Yoshinori Ohsumi
  • ,
  • Nobuo N Noda

77
6
開始ページ
1163
終了ページ
1175
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.molcel.2019.12.026

Clearance of biomolecular condensates by selective autophagy is thought to play a crucial role in cellular homeostasis. However, the mechanism underlying selective autophagy of condensates and whether liquidity determines a condensate's susceptibility to degradation by autophagy remain unknown. Here, we show that the selective autophagic cargo aminopeptidase I (Ape1) undergoes phase separation to form semi-liquid droplets. The Ape1-specific receptor protein Atg19 localizes to the surface of Ape1 droplets both in vitro and in vivo, with the "floatability" of Atg19 preventing its penetration into droplets. In vitro reconstitution experiments reveal that Atg19 and lipidated Atg8 are necessary and sufficient for selective sequestration of Ape1 droplets by membranes. This sequestration is impaired by mutational solidification of Ape1 droplets or diminished ability of Atg19 to float. Taken together, we propose that cargo liquidity and the presence of sufficient amounts of autophagic receptor on cargo are crucial for selective autophagy of biomolecular condensates.

リンク情報
DOI
https://doi.org/10.1016/j.molcel.2019.12.026
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31995729
ID情報
  • DOI : 10.1016/j.molcel.2019.12.026
  • PubMed ID : 31995729

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