2000年9月
Inorganic polyphosphate/ATP-NAD kinase of Micrococcus flavus and Mycobacterium tuberculosis H37Rv
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- 巻
- 276
- 号
- 1
- 開始ページ
- 57
- 終了ページ
- 63
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1006/bbrc.2000.3433
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
An enzyme with both inorganic polyphosphate [poly(P)]- and ATP-dependent NAD kinase activities was isolated from Micrococcus flavus. The enzyme was a dimer consisting of 34 kDa subunits, and was named poly(P)/ATP-NAD kinase. Internal amino acid sequences of the enzyme showed homologies with some function-unknown proteins released on the GenBank database. Among such proteins, hypothetical Rv1695 protein (Accession No. Z98268-16), which was encoded by a gene named "Rv1695" on genomic DNA of Mycobacterium tuberculosis H37Rv, was proposed to be poly(P)-dependent NAD kinase, By cloning and expression in Escherichia coli, Rv1695 was shown to encode poly(P)/ATP-NAD kinase and named ppnk. The ppnk product, recombinant-poly(P)/ATP-NAD kinase (Ppnk) was purified and characterized. The enzyme was a tetramaer consisting of 35 kDa subunits when expressed in E. coli. Poly(P)/ATP-NAD kinases of M. flavus and Ppnk of M; tuberculosis H37Rv specifically and completely phosphorylated NAD by utilizing commercially available poly(P)s and nucleoside triphosphates as phosphoryl donors. (C) 2000 Academic Press.
- リンク情報
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- DOI
- https://doi.org/10.1006/bbrc.2000.3433
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902115196391103
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11006082
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000089388100011&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1006/bbrc.2000.3433
- ISSN : 0006-291X
- eISSN : 1090-2104
- J-Global ID : 200902115196391103
- PubMed ID : 11006082
- Web of Science ID : WOS:000089388100011