2005年1月
Molecular identification of Sphingomonas sp A1 alginate lyase (A1-IV ') as a member of novel polysaccharide lyase family 15 and implications in alginate lyase evolution
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
- ,
- ,
- ,
- 巻
- 99
- 号
- 1
- 開始ページ
- 48
- 終了ページ
- 54
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1263/jbb.99.048
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
Sphingomonas sp. A1 (strain A1) produces three endotypes (A1-I [65 kDa], A1-II [25 kDa], and A1-III [40 kDa]) and an exotype (A1-IV [86 kDa]) alginate lyases in cytoplasm. These four enzymes cooperatively depolymerize alginate into constituent monosaccharides. In addition to the genes for these lyases, novel genes encoding hypothetical proteins homologous with A1-IV were found in the genomes of many bacteria including strain A1. One such protein, A1-IV'(90 kDa) of strain A1, was overexpressed in Escherichia coli cells, purified, and characterized. A1-IV' catalyzed the cleavage of glycosidic bonds in alginate through a beta-elimination reaction and released unsaturated di- and trisaccharides as main products, thus indicating that the enzyme is an endotype alginate lyase. A1-IV', which differed from A1-IV in some enzymatic properties, was not expressed in strain A1, suggesting that A1-IV' has no significant role in alginate metabolism. A1-IV' and other A1-IV homologs facilitate the creation of novel polysaccharide lyase family 15 based on their primary structures, implying the evolution route of alginate lyases in family PL-15.
- リンク情報
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- DOI
- https://doi.org/10.1263/jbb.99.048
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902221134899920
- CiNii Articles
- http://ci.nii.ac.jp/naid/110002695623
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16233753
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000227785600008&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1263/jbb.99.048
- ISSN : 1389-1723
- J-Global ID : 200902221134899920
- CiNii Articles ID : 110002695623
- PubMed ID : 16233753
- Web of Science ID : WOS:000227785600008