2013年3月
Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development
INTERNATIONAL IMMUNOLOGY
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- 巻
- 25
- 号
- 3
- 開始ページ
- 145
- 終了ページ
- 156
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/intimm/dxs095
- 出版者・発行元
- OXFORD UNIV PRESS
The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged immunoglobulin heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface immunoglobulin, while the B1-cell maintenance requires the peritoneal environment. The B1-cell expansion takes place in spleen, and is held in check by competitor B2 cells. The results indicate that the NT-ES method could replace the transgenic or knock-in mouse approaches currently used to study the biology of cells that undergo somatic rearrangements of their antigen receptor genes.
- リンク情報
- ID情報
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- DOI : 10.1093/intimm/dxs095
- ISSN : 0953-8178
- PubMed ID : 23042789
- Web of Science ID : WOS:000316291600002