論文

査読有り
2013年3月

Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development

INTERNATIONAL IMMUNOLOGY
  • Mitsuyo Takase
  • ,
  • Ryuji Iida
  • ,
  • Mikako Maruya
  • ,
  • Asako Sakaue-Sawano
  • ,
  • Atsushi Miyawaki
  • ,
  • Teruhiko Wakayama
  • ,
  • Satoshi Nishigami
  • ,
  • Sidonia Fagarasan
  • ,
  • Osami Kanagawa

25
3
開始ページ
145
終了ページ
156
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/intimm/dxs095
出版者・発行元
OXFORD UNIV PRESS

The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged immunoglobulin heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface immunoglobulin, while the B1-cell maintenance requires the peritoneal environment. The B1-cell expansion takes place in spleen, and is held in check by competitor B2 cells. The results indicate that the NT-ES method could replace the transgenic or knock-in mouse approaches currently used to study the biology of cells that undergo somatic rearrangements of their antigen receptor genes.

リンク情報
DOI
https://doi.org/10.1093/intimm/dxs095
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23042789
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000316291600002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/intimm/dxs095
  • ISSN : 0953-8178
  • PubMed ID : 23042789
  • Web of Science ID : WOS:000316291600002

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