In this report, the role of Magnesium on the crystal structure, morphology, and its luminescence proper-ties of Zn1xMgxO particle were investigated. The Zn1xMgxO particle was prepared by spray pyrolysismethod. The obtained particle was analyzed using XRD, SEM-EDS, and photoluminescence spectroscopycorrespondingly for crystal structure, morphology and elemental analysis, and photoluminescence prop-erties due to the presence of magnesium dopant. The XRD results showed that the original structure ofthe particle is hexagonal wurtzite and change to cubical as the increase of Mg. Thea-lattice parameterincreased, andc-lattice parameter decreased with increasing the concentration of Mg in wurtzite crystal.The SEM results exhibited different morphology of the resulting particle. The morphology of the sample isdense with wrinkled shape and is changing to spherical as the result of Mg incorporation into the crystal.Photoluminescence study revealed that the addition of Mg affects the existence of new energy statebeside native defect of ZnO in between valence and conduction band. The widening of bandgap onZn1xMgxO is due to the blue shift of excitonic peak in the excitation spectrum that usually calledBurstein-Moss effect