論文

査読有り 本文へのリンクあり
2015年9月

Decoding enhancers using massively parallel reporter assays

Genomics
  • Fumitaka Inoue
  • ,
  • Nadav Ahituv

担当区分
筆頭著者
106
3
開始ページ
159
終了ページ
164
DOI
10.1016/j.ygeno.2015.06.005

© 2015 Elsevier Inc. Enhancers control the timing, location and expression levels of their target genes. Nucleotide variation in enhancers has been shown to lead to numerous phenotypes, including human disease. While putative enhancer sequences and nucleotide variation within them can now be detected in a rapid manner using various genomic technologies, the understanding of the functional consequences of these variants still remains largely unknown. Massively parallel reporter assays (MPRAs) can overcome this hurdle by providing the ability to test thousands of sequences and nucleotide variants within them for enhancer activity en masse. Here, we describe this technology and specifically focus on how it is being used to obtain an increased understanding of enhancer regulatory code and grammar.

リンク情報
DOI
https://doi.org/10.1016/j.ygeno.2015.06.005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26072433
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540663
URL
http://europepmc.org/abstract/med/26072433
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84939255435&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84939255435&origin=inward

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