1999年10月
Identification of the human sperm protein that interacts with sperm-immobilizing antibodies in the sera of infertile women
FERTILITY AND STERILITY
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- 巻
- 72
- 号
- 4
- 開始ページ
- 691
- 終了ページ
- 695
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0015-0282(99)00314-3
- 出版者・発行元
- ELSEVIER SCIENCE INC
Objective: To identify the target antigen of sperm-immobilizing antibodies present in the circulation of infertile women.
Design: Laboratory research.
Setting: Academic research laboratory.
Patient(s): Twenty-nine infertile women with sperm-immobilizing antibodies, 22 infertile women with other disorders, and 20 fertile women.
Intervention(s): Titers of antibodies to the sperm protein, rSMP-B, were determined by ELISA using as Substrate the synthetic peptide segment (rSMP-230) that corresponds with the hydrophilic domain of rSMP-B. Tests for sperm immobilization and zona pellucida penetration were performed using the human IVF system.
Main Outcome Measure(s): Human sera with sperm-immobilizing activity were assayed for the presence of antibodies to rSMP-230. Polyclonal antibodies to rSMP-230 were assessed for the same biologic activities as sperm-immobilizing antibodies.
Result(s): Antibodies to rSMP-230 were detected in 10 (34%) of 29 sera obtained from women with immunologic infertility. In contrast, only one serum sample (2%) from women without sperm-immobilizing activity had a low titer of antibodies to rSMP-230. Polyclonal antibodies to rSMP-230 completely immobilized human sperm in the presence of complement and blocked sperm penetration across the zona pellucida.
Conclusion(s): The human sperm protein, rSMP-B, probably is the target antigen of sperm-immobilizing antibodies. (Fertil Steril(R) 1999;72:691-5. (C)1999 by American Society for Reproductive Medicine.).
Design: Laboratory research.
Setting: Academic research laboratory.
Patient(s): Twenty-nine infertile women with sperm-immobilizing antibodies, 22 infertile women with other disorders, and 20 fertile women.
Intervention(s): Titers of antibodies to the sperm protein, rSMP-B, were determined by ELISA using as Substrate the synthetic peptide segment (rSMP-230) that corresponds with the hydrophilic domain of rSMP-B. Tests for sperm immobilization and zona pellucida penetration were performed using the human IVF system.
Main Outcome Measure(s): Human sera with sperm-immobilizing activity were assayed for the presence of antibodies to rSMP-230. Polyclonal antibodies to rSMP-230 were assessed for the same biologic activities as sperm-immobilizing antibodies.
Result(s): Antibodies to rSMP-230 were detected in 10 (34%) of 29 sera obtained from women with immunologic infertility. In contrast, only one serum sample (2%) from women without sperm-immobilizing activity had a low titer of antibodies to rSMP-230. Polyclonal antibodies to rSMP-230 completely immobilized human sperm in the presence of complement and blocked sperm penetration across the zona pellucida.
Conclusion(s): The human sperm protein, rSMP-B, probably is the target antigen of sperm-immobilizing antibodies. (Fertil Steril(R) 1999;72:691-5. (C)1999 by American Society for Reproductive Medicine.).
- リンク情報
- ID情報
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- DOI : 10.1016/S0015-0282(99)00314-3
- ISSN : 0015-0282
- Web of Science ID : WOS:000082851200022