2005年11月
Unique features of a pH-sensitive fusogenic peptide that improves the transfection efficiency of cationic liposomes
JOURNAL OF GENE MEDICINE
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- 巻
- 7
- 号
- 11
- 開始ページ
- 1450
- 終了ページ
- 1458
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jgm.796
- 出版者・発行元
- JOHN WILEY & SONS LTD
Background One of the critical steps in intracellular gene delivery using cationic liposomes is the endosomal escape of the plasmid/liposome complexes to the cytosol. The addition of GALA, a pH-sensitive fusogenic peptide, is a promising method to accelerate this step in order to enhance the expression of the desired proteins. Detailed studies on the methods of enhancement would broaden the horizon of its application.
Methods Using representative commercially available cationic liposomes (Lipofectin, Lipofectamine, and Lipofectamine 2000), the effects of GALA on transfection. efficiency were studied by luciferase assay and confocal microscopic observations.
Results A concentration-dependent increase in the transfection efficiency was observed for GALA. Addition of 0.1 mu M GALA to the plasmid/liposome complex significantly increased the transfection efficiency, especially in the case of Lipofectin, but higher concentration of GALA decreased transfection efficiency. Successful reduction in the liposomal dosage was attained by employing GALA while maintaining a high transfection efficiency. Interestingly, although the transfection efficiency was higher in the presence of GALA, a lower amount of the plasmid DNA was taken up by the cells. Confocal microscopic observations of the rhodamine-labeled plasmid did not show a significant difference in the cellular localization among cells incubated in the presence or absence of GALA, suggesting that a slight increase in GALA-induced release of the plasmid to the cytosol may cause a significant change in the transfection efficiency.
Conclusion The unique features of GALA to mediate improved transfection efficiencies were identified. Copyright (C) 2005 John Wiley & Sons, Ltd.
Methods Using representative commercially available cationic liposomes (Lipofectin, Lipofectamine, and Lipofectamine 2000), the effects of GALA on transfection. efficiency were studied by luciferase assay and confocal microscopic observations.
Results A concentration-dependent increase in the transfection efficiency was observed for GALA. Addition of 0.1 mu M GALA to the plasmid/liposome complex significantly increased the transfection efficiency, especially in the case of Lipofectin, but higher concentration of GALA decreased transfection efficiency. Successful reduction in the liposomal dosage was attained by employing GALA while maintaining a high transfection efficiency. Interestingly, although the transfection efficiency was higher in the presence of GALA, a lower amount of the plasmid DNA was taken up by the cells. Confocal microscopic observations of the rhodamine-labeled plasmid did not show a significant difference in the cellular localization among cells incubated in the presence or absence of GALA, suggesting that a slight increase in GALA-induced release of the plasmid to the cytosol may cause a significant change in the transfection efficiency.
Conclusion The unique features of GALA to mediate improved transfection efficiencies were identified. Copyright (C) 2005 John Wiley & Sons, Ltd.
- リンク情報
- ID情報
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- DOI : 10.1002/jgm.796
- ISSN : 1099-498X
- PubMed ID : 16025556
- Web of Science ID : WOS:000233578300008