2013年9月20日
Curvature engineering: positive membrane curvature induced by epsin N-terminal peptide boosts internalization of octaarginine.
ACS chemical biology
- 巻
- 8
- 号
- 9
- 開始ページ
- 1894
- 終了ページ
- 9
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/cb4002987
Epsin-1 is a representative protein for inducing the positive curvature necessary for the formation of clathrin-coated pits. Here we demonstrate that the N-terminus 18-residue peptide of epsin-1 (EpN18) has this ability per se, as proved by differential scanning calorimetry (DSC) and solid-state NMR. Moreover, it is shown how this positive curvature promotion can be exploited for promoting the direct penetration of a representative cell-penetrating peptide (CPP), octaarginine (R8), through artificial and plasma membranes. This synergistic effect has been used for the efficient delivery of a proapoptotic domain peptide (PAD), which induced high level of apoptosis only when coadministered with R8 and EpN18, thus emphasizing the importance of positive curvature induction for achieving the desired ultimate cargo bioavailability.
- リンク情報
- ID情報
-
- DOI : 10.1021/cb4002987
- ISSN : 1554-8929
- PubMed ID : 23834464
- Web of Science ID : WOS:000330016700007