Makoto Fujisawa - My portal

Profile Information

Affiliation: Toyo University

Degree: 博士(生命科学)

Researcher number: 70549641
J-GLOBAL ID: 201301071633181670
researchmap Member ID: B000231508

External link: http://kaken.nii.ac.jp/d/r/70549641.ja.html

Research Interests

Research Areas

Research History

Apr, 2013 - Present

東洋大学食環境科学部健康栄養学科准教授
Apr, 2010 - Mar, 2013

Faculty of Life Sciences, Toyo University
Nov, 2008 - Mar, 2009

東洋大学バイオ・ナノエレクトロニクス研究センター研究助手
Apr, 2006 - Oct, 2008

Department of Systems Therapeutics, Mount Sinai School of Medicine, New York, NY, USA 博士研究員

Education

Awards

2008

第9回極限環境微生物学会年会ポスター賞受賞

藤澤誠
2005

東洋大学校友会奨学生

藤澤誠

Papers

Killing effect of peracetic acid to Paenibacillus favisporus and P. chibensis spores isolated from soft drink manufacturing factory

Jun Sato, Yu Sato, Makoto Fujisawa, Mana Takano, Chisato Hoozumi

Journal of Antibacterial and Antifungal Agents, 45(9) 431-436, Sep, 2017 Peer-reviewed
The ATP Synthase a-subunit of Extreme Alkaliphiles Is a Distinct Variant MUTATIONS IN THE CRITICAL ALKALIPHILE-SPECIFIC RESIDUE LYS-180 AND OTHER RESIDUES THAT SUPPORT ALKALIPHILE OXIDATIVE PHOSPHORYLATION

Makoto Fujisawa, Oliver J. Fackelmayer, Jun Liu, Terry A. Krulwich, David B. Hicks

JOURNAL OF BIOLOGICAL CHEMISTRY, 285(42) 32105-32115, Oct, 2010
F1F0-ATP synthases of alkaliphilic bacteria: Lessons from their adaptations

David B. Hicks, Jun Liu, Makoto Fujisawa, Terry A. Krulwich

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1797(8) 1362-1377, Aug, 2010
Industrial applications of alkaliphiles and their enzymes - past, present and future

Shun Fujinami, Makoto Fujisawa

ENVIRONMENTAL TECHNOLOGY, 31(8-9) 845-856, 2010
Characterization of Bacillus subtilis YfkE (ChaA): a calcium-specific Ca2+/H+ antiporter of the CaCA family

Makoto Fujisawa, Yuko Wada, Takahiro Tsuchiya, Masahiro Ito

ARCHIVES OF MICROBIOLOGY, 191(8) 649-657, Aug, 2009
Characterization of the Functionally Critical AXAXAXA and PXXEXXP Motifs of the ATP Synthase c-Subunit from an Alkaliphilic Bacillus

Jun Liu, Makoto Fujisawa, David B. Hicks, Terry A. Krulwich

JOURNAL OF BIOLOGICAL CHEMISTRY, 284(13) 8714-8725, Mar, 2009
Cytoplasmic pH Measurement and Homeostasis in Bacteria and Archaea

Joan L. Slonczewski, Makoto Fujisawa, Mark Dopson, Terry A. Krulwich

ADVANCES IN MICROBIAL PHYSIOLOGY, VOL 55, 55 1-79, 2009
Channel-like two-component transporters that have monovalent-cation/proton antiport activity

FUJISAWA Makoto, ITO Masahiro

Bioscience & industry, 66(6) 300-302, Jun 1, 2008
Three two-component transporters with channel-like properties have monovalent cation/proton antiport activity

Makoto Fujisawa, Masahiro Ito, Terry A. Krulwich

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104(33) 13289-13294, Aug, 2007
An intergenic stem-loop mutation in the Bacillus subtilis ccpA-motPS operon increases motPS transcription and the MotPS contribution to motility

N Terahara, M Fujisawa, B Powers, TM Henkin, TA Krulwich, M Ito

JOURNAL OF BACTERIOLOGY, 188(7) 2701-2705, Apr, 2006
NhaK, a novel monovalent cation/H+ antiporter of Bacillus subtilis

M Fujisawa, A Kusumoto, Y Wada, T Tsuchiya, M Ito

ARCHIVES OF MICROBIOLOGY, 183(6) 411-420, Sep, 2005
Modulation of the K+ efflux activity of Bacillus subtilis YhaU by YhaT and the C-terminal region of YhaS

M Fujisawa, Y Wada, M Ito

FEMS MICROBIOLOGY LETTERS, 231(2) 211-217, Feb, 2004

Misc.

Adaptations of the ATP synthase a-subunit to support synthesis at low protonmotive force at both pH 7.5 and 10.5 may underpin the more stringent requirement for lysine-180 in TMH-4 by alkaliphilic Bacillus pseudofirmus OF4 than by more modestly alkaliphilic thermoalkaliphile Bacillus sp TA2.A1

Makoto Fujisawa, Jun Liu, Oliver Fackelmayer, Terry A. Krulwich, David B. Hicks

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1797 33-34, Jul, 2010
Special adaptations of the ATP synthase F-o sector are required for alkaliphile oxphos at high pH

David B. Hicks, Jun Liu, Makoto Fujisawa, Terry A. Krulwich

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1777 S16-S16, Jul, 2008
Substitutions of the alkaliphile-specific Lys(180) in the putative proton uptake pathway of the a-subunit of F1F0-ATP synthase do not support function at pH 10.5

Makoto Fujisawa, David B. Hicks, Terry A. Krulwich

BIOPHYSICAL JOURNAL, 640A-640A, Jan, 2007

Books and Other Publications

栄養科学イラストレイテッド微生物学

大橋典男, 藤澤誠 (Role: Contributor, 第2章微生物の制御 1. 微生物の制御とは 2. 微生物の増殖条件)

羊土社, Mar, 2020 (ISBN: 9784758113588)
高濃度のリチウムイオン存在下で酸化的リン酸化によって生育する好アルカリ性細菌の単離とゲノムから予測される生理的性質

藤澤誠

極限環境生物学会・Journal of Japanese Society for Extremophiles, Dec 1, 2012
Pt 2.6 Adaptive Mechanisms of Extreme Alkaliphiles; Extremophile Handbook

藤澤誠

Springer-Verlag, Dec 10, 2010
1価カチオン/プロトン対向輸送活性を持つチャネル様二成分系トランスポーター

藤澤誠, 伊藤政博

バイオサイエンスとインダストリー Vol. 66 No. 6, Jun 1, 2008
2成分K+/H+アンチポーターの発見 - アンチポーター(2次的能動輸送)とチャネル(受動輸送)にまたがるタンパク質

藤澤誠

化学と生物 Vol. 46 No. 5, May 1, 2008

Presentations

Characterization of germination and surface properties of Paenibacillus chibensis PB-434 spores

Mami Kageyama, Yu Sato, Jun Sato, Makoto Fujisawa

20th International Conference on Bacilli and Gram-Positive Bacteria
Paenibacillus chibensis PB-434株の胞子の過酢酸耐性機構に関する研究

田邉廣大, 齋藤隆志, 佐藤順, 藤澤誠

第36回（2013年）日本分子生物学会年会, Dec, 2013
The interactions between membrane proteins from Escherichia coli and nanomaterials

Yuzo Ishihara, Mio Kojima, Akira Inoue, Makoto Fujisawa

Dec, 2012
Purification of YhaTU system,a K+/H+ antiporter from Bacillus subtilis

Kentaro Kato, Yu Tanaami, Makoto Fujisawa

Dec, 2012
The genome analysis of Bacillus pseudofirmus KU50 isolated from commercially available salt.

Makoto Fujisawa, Kazuaki Odakura, Manato Watanabe, Takuya Kamiyama, Kazuya Noda

Dec, 2012
Isolation of sodium non-requirement alkaliphiles and its physiology predicted from comparative genome analysis with sodium dependent ones.

藤澤誠, 小田倉和明, 渡邉真名斗, 神山卓也, 野田和也

9th INTERNATIONAL CONGRESS ON EXTREMOPHILES, Sep 13, 2012
ゲノム解析から見えてきたナトリウムイオン非依存性の好アルカリ性細菌の環境適応機構

藤澤誠

極限環境生物学会第13回シンポジウム, Jun 23, 2012
Detection of YhaTU complex, a Two-Component K+/H+ Antiporter from Bacillus subtilis, by Pull-down Assay and Blue Native PAGE.

藤澤誠

9th International Symposium on Bioscience and Nanotechnology, Dec 10, 2011
プルダウンアッセイとブルーネイティブゲル電気泳動法による枯草菌のK+/H+アンチポーターYhaTUシステムの複合体の検出

藤澤誠

日本農芸化学会関東支部2011年度大会, Oct 15, 2011
Detection of YhaTU complex, a Two-Component K+/H+ Antiporter from Bacillus subtilis, by Pull-down Assay and Blue Native PAGE.

藤澤誠

International Union of Microbiological Societies 2011 Congress., Sep 6, 2011
Detection of interaction between subunits of a two-component K+/H+ antiporter, YhaTU, from Bacillus subtilis.

藤澤誠

Gordon Research Conference – Mechanisms of Membrane Transport, Jun 19, 2011
CPA2ファミリーに属する2成分K+/H+アンチポーターのサブユニット間の相互作用の検出

藤澤誠

第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(神戸), Dec 7, 2010
低濃度のナトリウムイオン存在下で生育する好アルカリ性細菌の単離と生理的性質

藤澤誠

第11回極限環境生物学会年会(京都), Nov 16, 2010

Teaching Experience

Professional Memberships

Research Projects

2成分K+/H+アンチポーターYhaTUの大量精製系の確立と作用機序の解明

科学研究費若手研究（Ｂ）, 2011 - 2013

藤澤誠
新しく単離されたNa+非依存性好アルカリ性細菌Bacillus pseudofirmus KU50株のアルカリ環境適応機構の解明

特別研究（個人研究）, 2011 - 2011

藤澤誠
大腸菌と枯草菌で見つかった新規2成分K+/H+アンチポーターの作用機序の解明

科学研究費若手研究（スタートアップ）, 2009 - 2010

藤澤誠