Jun, 2018 - Mar, 2020
Elucidation of undifferentiated maintaining mechanism of stem cell towards the control of stem cell aging
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory) Grant-in-Aid for Challenging Research (Exploratory)
- Grant number
- 18K19646
- Japan Grant Number (JGN)
- JP18K19646
- Grant amount
-
- (Total)
- 6,240,000 Japanese Yen
- (Direct funding)
- 4,800,000 Japanese Yen
- (Indirect funding)
- 1,440,000 Japanese Yen
Stem cells are required for lifelong homeostasis and regeneration of organs and tissues in mammals. However, aging of stem cells reduces cellular function and results in dysfunctional organs and tissues. Therefore, maintenance of the stemness of stem cells is of crucial importance, although its mechanisms are still unclear. The aim of this study was to identify the master regulator for the maintenance of stemness of bone marrow mesenchymal stem cells (BMSCs). First, we compared the RNA expression patterns between BMSCs derived from young and old mice, as well as between human BMSCs and human dermal fibroblasts using RNA-seq, and found that 30 transcription factors were highly expressed in BMSCs derived from young mice and in human BMSCs. Next, we found that several transcription factors could inhibit the induction to pluripotent stem (iPS) cells using iPS interfering method. The detailed function of these transcription factors in BMSCs are now being investigated.
- Link information
- ID information
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- Grant number : 18K19646
- Japan Grant Number (JGN) : JP18K19646