Apr, 2017 - Mar, 2021
Molecular understanding of the accumulation of mesenchymal stem cells in vivo by photo-manipulation techniques and its application to bone-related diseases
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)
- Grant number
- 17H04399
- Japan Grant Number (JGN)
- JP17H04399
- Grant amount
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- (Total)
- 16,640,000 Japanese Yen
- (Direct funding)
- 12,800,000 Japanese Yen
- (Indirect funding)
- 3,840,000 Japanese Yen
In this study, we generated a tTA-dependent photoactivatable Cre-loxP recombinase knock-in mouse model (TRE-PA-Cre mice) using a CRISPR/Cas9 system. These mice were crossed with ROSA26-tdTomato mice (Cre reporter mouse) to visualize DNA recombination as marked by tdTomato expression. We demonstrated that external noninvasive LED blue light illumination allows efficient DNA recombination in the liver of TRE-PA-Cre:ROSA26-tdTomato mice transfected with tTA expression vectors using hydrodynamic tail vein injection. The TRE-PA-Cre mouse established here promises to be useful for optogenetic genome engineering in a noninvasive, spatiotemporal, and cell-type specific manner in vivo.
- Link information
- ID information
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- Grant number : 17H04399
- Japan Grant Number (JGN) : JP17H04399