In order to examine the seasonal and spatial distributions of benthic animals in the intertidal mudflat of the southern Yellow River Delta, field investigations were carried out in 2007 and 2008 and multiple methods were applied. Results showed that, the biomass of macro benthos ranged at 0.75-1151.00 g wet m(-2) and averaged at 156.31 g wet m(-2), in which Mactra veneriformis accounted for 75.6%-93.4% of the total macro benthic biomass. More than 90% of macro benthos inhabited in the middle and low tide lines, and higher biomass occurred in early summer and lower in winter. Statistical analysis showed that: 1) M. veneriformis growth was primarily favored at higher temperature and lower salinity; 2) after long time interaction, benthic bivalve grazers led to patching distributions of Chlorophyll a (Chl a); 3) macro benthic biomass positively related with Chl a when the concentration of Chl a was low, but they were negatively related when Chl a concentration was high; and 4) furthermore, the biomass of benthic bivalves peaked in the sediment with median grain size about 0.55 mm, but decreased gradually in coarse or fine sediments. The secondary productivity ranged at 0.37-283.68 g m(-2)yr(-1) and averaged at 47.88 g m(-2) yr(-1), in which 69.7% was contributed by M. veneriformis It was estimated that primary production was transformed to secondary production at a rate of 6.87% approximately, which implies that there is a local sustainability of high bivalve production.