2016年8月15日
AP-1 family members act with Sox9 to promote chondrocyte hypertrophy.
Development (Cambridge, England)
- ,
- ,
- ,
- 巻
- 143
- 号
- 16
- 開始ページ
- 3012
- 終了ページ
- 23
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1242/dev.134502
- 出版者・発行元
- COMPANY OF BIOLOGISTS LTD
An analysis of Sox9 binding profiles in developing chondrocytes identified marked enrichment of an AP-1-like motif. Here, we have explored the functional interplay between Sox9 and AP-1 in mammalian chondrocyte development. Among AP-1 family members, Jun and Fosl2 were highly expressed within prehypertrophic and early hypertrophic chondrocytes. Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) showed a striking overlap in Jun- and Sox9-bound regions throughout the chondrocyte genome, reflecting direct binding of each factor to the same enhancers and a potential for protein-protein interactions within AP-1- and Sox9-containing complexes. In vitro reporter analysis indicated that direct co-binding of Sox9 and AP-1 at target motifs promoted gene activity. By contrast, where only one factor can engage its DNA target, the presence of the other factor suppresses target activation consistent with protein-protein interactions attenuating transcription. Analysis of prehypertrophic chondrocyte removal of Sox9 confirmed the requirement of Sox9 for hypertrophic chondrocyte development, and in vitro and ex vivo analyses showed that AP-1 promotes chondrocyte hypertrophy. Sox9 and Jun co-bound and co-activated a Col10a1 enhancer in Sox9 and AP-1 motif-dependent manners consistent with their combined action promoting hypertrophic gene expression. Together, the data support a model in which AP-1 family members contribute to Sox9 action in the transition of chondrocytes to the hypertrophic program.
- リンク情報
-
- DOI
- https://doi.org/10.1242/dev.134502
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/27471255
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5004882
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000393450300014&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84983085532&partnerID=MN8TOARS
- ID情報
-
- DOI : 10.1242/dev.134502
- ISSN : 0950-1991
- eISSN : 1477-9129
- ORCIDのPut Code : 84496222
- PubMed ID : 27471255
- PubMed Central 記事ID : PMC5004882
- SCOPUS ID : 84983085532
- Web of Science ID : WOS:000393450300014