2016年5月9日
Sp7/Osterix Is Restricted to Bone-Forming Vertebrates where It Acts as a Dlx Co-factor in Osteoblast Specification.
Developmental cell
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- ,
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- 巻
- 37
- 号
- 3
- 開始ページ
- 238
- 終了ページ
- 53
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.devcel.2016.04.002
- 出版者・発行元
- CELL PRESS
In extant species, bone formation is restricted to vertebrate species. Sp7/Osterix is a key transcriptional determinant of bone-secreting osteoblasts. We performed Sp7 chromatin immunoprecipitation sequencing analysis identifying a large set of predicted osteoblast enhancers and validated a subset of these in cell culture and transgenic mouse assays. Sp family members bind GC-rich target sequences through their zinc finger domain. Several lines of evidence suggest that Sp7 acts differently, engaging osteoblast targets in Dlx-containing regulatory complexes bound to AT-rich motifs. Amino acid differences in the Sp7 zinc finger domain reduce Sp7's affinity for the Sp family consensus GC-box target; Dlx5 binding maps to this domain of Sp7. The data support a model in which Dlx recruitment of Sp7 to osteoblast enhancers underlies Sp7-directed osteoblast specification. Because an Sp7-like zinc finger variant is restricted to vertebrates, the emergence of an Sp7 member within the Sp family was likely closely coupled to the evolution of bone-forming vertebrates.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.devcel.2016.04.002
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/27134141
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964983
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000375573300009&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.devcel.2016.04.002
- ISSN : 1534-5807
- eISSN : 1878-1551
- PubMed ID : 27134141
- PubMed Central 記事ID : PMC4964983
- Web of Science ID : WOS:000375573300009