2017年3月
Epigenetic Regulation of Intronic Transgenes in Arabidopsis
SCIENTIFIC REPORTS
- ,
- ,
- ,
- 巻
- 7
- 号
- 開始ページ
- 45166
- 終了ページ
- 45166
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep45166
- 出版者・発行元
- NATURE PUBLISHING GROUP
Defense mechanisms of plant genomes can epigenetically inactivate repetitive sequences and exogenous transgenes. Loss of mutant phenotypes in intronic T-DNA insertion lines by interaction with another T-DNA locus, termed T-DNA suppression, has been observed in Arabidopsis thaliana, although the molecular basis of establishment and maintenance of T-DNA suppression is poorly understood. Here we show that maintenance of T-DNA suppression requires heterochromatinisation of T-DNA sequences and the nuclear proteins, INCREASED IN BONSAI METHYLATION 2 (IBM2) and ENHANCED DOWNY MILDEW 2 (EDM2), which prevent ectopic 3' end processing of mRNA in atypically long introns containing T-DNA sequences. Initiation of T-DNA suppression is mediated by the canonical RdDM pathway after hybridisation of two T-DNA strains, accompanied by DNA hypermethylation of T-DNA sequences in the F1 generation. Our results reveal the presence of a genome surveillance mechanism through genome hybridisation that masks repetitive DNAs intruding into transcription units.
- リンク情報
-
- DOI
- https://doi.org/10.1038/srep45166
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28338020
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364540
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397380900001&DestApp=WOS_CPL
- URL
- http://europepmc.org/abstract/med/28338020
- URL
- http://orcid.org/0000-0002-8647-1430
- ID情報
-
- DOI : 10.1038/srep45166
- ISSN : 2045-2322
- ORCIDのPut Code : 31482055
- PubMed ID : 28338020
- PubMed Central 記事ID : PMC5364540
- Web of Science ID : WOS:000397380900001