2016年
Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis
Bioscience Biotechnology and Biochemistry
- 巻
- 80
- 号
- 10
- 開始ページ
- 2051
- 終了ページ
- 2057
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1080/09168451.2016.1189320
- 出版者・発行元
- TAYLOR & FRANCIS LTD
Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately -1 degrees Cmin(-1) in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately -1 degrees Cmin(-1) in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains.
- リンク情報
- ID情報
-
- DOI : 10.1080/09168451.2016.1189320
- ISSN : 0916-8451
- eISSN : 1347-6947
- Web of Science ID : WOS:000383046300029