論文

査読有り
1992年12月

Efficient transformation of Arabidopsis thaliana: Comparison of the efficiencies with various organs, plant ecotypes and Agrobacterium strains

Plant Cell Reports
  • K Akama
  • ,
  • H Shiraishi
  • ,
  • S Ohta
  • ,
  • K Nakamura
  • ,
  • K Okada
  • ,
  • Y Shimura

12
1
開始ページ
7
終了ページ
11
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/BF00232413
出版者・発行元
SPRINGER VERLAG

The efficiency of Agrobacterium-mediated transformation of Arabidopsis thaliana was compared with different organs, Arabidopsis ecotypes, and Agrobacteriwn strains. Efficiency of shoot regeneration was examined using hypocotyl, cotyledon and root explants prepared from young seedlings. Hypocotyl explants had the highest regeneration efficiency in all of the four Arabidopsis ecotypes tested, when based on a tissue culture system of callus-inducing medium (CIM: Valvekens et al. 1988) and shoot-inducing medium (SIM: Feldmann and Marks 1986). Histochemical analysis using the beta-glucuronidase (GUS) reporter gene showed that the gusA gene expression increased as the period of preincubation on CIM was extended, suggesting that dividing cells are susceptible to Agrobacteriwn infection. In order to obtain transgenic shoots, hypocotyl explants preincubated for 7 or 8 days on CIM were infected with Agrobacteriwn containing a binary vector which carries two drug-resistant genes as selection markers, and transferred to SIM for selection of transformed shoots. Of four Arabidopsis ecotypes and of three Agrobacteriwn strains examined, Wassilewskija ecotype and EHA101 strain showed the highest efficiency of regeneration of transformed shoots. By combining the most efficient factors of preincubation period, Arabidopsis ecotype, tissue, and bacterial strain, we obtained a transformation efficiency of about 80-99%. Southern analysis of 124 transgenic plants showed that 44% had one copy of inserted T-DNA while the others had more than one copy.

リンク情報
DOI
https://doi.org/10.1007/BF00232413
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1992KB51900002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/BF00232413
  • ISSN : 0721-7714
  • Web of Science ID : WOS:A1992KB51900002

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