論文

査読有り
1997年10月

Identification of the nucleotides in the A-rich bulge of the Tetrahymena ribozyme responsible for an efficient self-splicing reaction

Journal of Biochemistry
  • Y Ikawa
  • ,
  • A Okada
  • ,
  • H Imahori
  • ,
  • H Shiraishi
  • ,
  • T Inoue

122
4
開始ページ
878
終了ページ
882
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
JAPANESE BIOCHEMICAL SOC

P5abc is a large extension of the PS element characteristic of subclasses IC1 and IC2 of group I introns. It has a conserved region termed the A-rich bulge, that is responsible for activation of the Tetrahymena self-splicing intron. By employing a modified color-colony assay system, we identified four adenosines in the bulge that are responsible for an efficient splicing reaction. On comparison with the,X-ray crystal structure of the P4-5-6 domains of the Tetrahymena intron, three adenosines at positions 183, 184, and 186 were found to be identical to those significantly contributing to the formation of its tertiary structure, However, our results show that an adenosine at 187 is involved in the formation of a Watson-Crick base pair with U135, although it forms a Hoogsteen base pair in the crystal structure.

リンク情報
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902165887633054
CiNii Articles
http://ci.nii.ac.jp/naid/10005840117
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/9399595
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997YA00500029&DestApp=WOS_CPL
ID情報
  • ISSN : 0021-924X
  • J-Global ID : 200902165887633054
  • CiNii Articles ID : 10005840117
  • PubMed ID : 9399595
  • Web of Science ID : WOS:A1997YA00500029

エクスポート
BibTeX RIS