1997年10月
Identification of the nucleotides in the A-rich bulge of the Tetrahymena ribozyme responsible for an efficient self-splicing reaction
Journal of Biochemistry
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- 巻
- 122
- 号
- 4
- 開始ページ
- 878
- 終了ページ
- 882
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- JAPANESE BIOCHEMICAL SOC
P5abc is a large extension of the PS element characteristic of subclasses IC1 and IC2 of group I introns. It has a conserved region termed the A-rich bulge, that is responsible for activation of the Tetrahymena self-splicing intron. By employing a modified color-colony assay system, we identified four adenosines in the bulge that are responsible for an efficient splicing reaction. On comparison with the,X-ray crystal structure of the P4-5-6 domains of the Tetrahymena intron, three adenosines at positions 183, 184, and 186 were found to be identical to those significantly contributing to the formation of its tertiary structure, However, our results show that an adenosine at 187 is involved in the formation of a Watson-Crick base pair with U135, although it forms a Hoogsteen base pair in the crystal structure.
- リンク情報
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- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902165887633054
- CiNii Articles
- http://ci.nii.ac.jp/naid/10005840117
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/9399595
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997YA00500029&DestApp=WOS_CPL
- ID情報
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- ISSN : 0021-924X
- J-Global ID : 200902165887633054
- CiNii Articles ID : 10005840117
- PubMed ID : 9399595
- Web of Science ID : WOS:A1997YA00500029