1999年4月
Trans-activation of the Tetrahymena group I intron ribozyme via a non-native RNA-RNA interaction
Nucleic Acids Research
- ,
- ,
- 巻
- 27
- 号
- 7
- 開始ページ
- 1650
- 終了ページ
- 1655
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/nar/27.7.1650
- 出版者・発行元
- OXFORD UNIV PRESS
The peripheral P2.1 domain of the Tetrahymena group I intron ribozyme has been shown to be non-essential for splicing. We found, however, that separately prepared P2.1 RNA efficiently accelerates the 3' splice-site-specific hydrolysis reaction of a mutant ribozyme lacking both P2.1 and its upstream region in trans. We report here the unusual properties of this transactivation. Compensatory mutational analysis revealed that non-native long-range base-pairings between the loop region of P2.1 RNA and L5c region of the mutant ribozyme are needed for the activation in spite of the fact that P2.1 forms base-pairings with P9.1 in the Tetrahymena ribozyme. The trans-activation depends on the non-native RNA-RNA interaction together with the higher order structure of P2.1 RNA. This activation is unique among the known trans-activations that utilize native tertiary interactions or RNA chaperons.
- リンク情報
- ID情報
-
- DOI : 10.1093/nar/27.7.1650
- ISSN : 0305-1048
- PubMed ID : 10075996
- Web of Science ID : WOS:000079504600011