Papers

Peer-reviewed Lead author Corresponding author
Nov, 2008

Novel Variants of Oct-3/4 Gene Expressed in Mouse Somatic Cells

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Nobuhiko Mizuno
  • ,
  • Mitsuko Kosaka

Volume
283
Number
45
First page
30997
Last page
31004
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1074/jbc.M802992200
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

It has been suggested that Oct-3/4 may regulate self-renewal in somatic stem cells, as it does in embryonic stem cells. However, recent reports raise the possibility that detection of human Oct-3/4 expression by RT-PCR is prone to artifacts generated by pseudogene transcripts and argue against a role for Oct-3/4 in somatic cells. In this study, we clarified Oct-3/4 expression in mouse somatic tissues using designed PCR primers, which can exclude amplification of its pseudogenes. We found that novel alternative transcripts are indeed expressed in somatic tissues, rather than the normal length transcripts in germline and ES cells. The alternative transcripts indicate the expression of two kinds of truncated proteins. Furthermore, we determined novel promoter regions that are sufficient for the expression of Oct-3/4 transcript variants in somatic cells. These findings provide new insights into the postnatal role of Oct-3/4 in somatic tissues.


Link information
DOI
https://doi.org/10.1074/jbc.M802992200
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000260544800054&DestApp=WOS_CPL
URL
http://orcid.org/0000-0001-6146-5184
ID information
  • DOI : 10.1074/jbc.M802992200
  • ISSN : 0021-9258
  • ORCID - Put Code : 26053918
  • Web of Science ID : WOS:000260544800054

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