Papers

Peer-reviewed Corresponding author
Apr, 2007

Multipotent cells from mammalian iris pigment epithelium

DEVELOPMENTAL BIOLOGY
  • Maki Asami
  • ,
  • Guangwei Sun
  • ,
  • Masahiro Yamaguchi
  • ,
  • Mitsuko Kosaka

Volume
304
Number
1
First page
433
Last page
446
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.ydbio.2006.12.047
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE

The regeneration of lens tissue from the iris of newts has become a classical model of developmental plasticity, although little is known about the corresponding plasticity of the mammalian iris. We here demonstrate and characterize multipotent cells within the iris pigment epithelium (IPE) of postnatal and adult rodents. Acutely-isolated IPE cells were morphologically homogeneous and highly pigmented, but some produced neurospheres which expressed markers characteristic of neural stem/progenitor cells. Stem/progenitor cell markers were also expressed in the IPE in vivo both neonatally and into adulthood. Inner and outer WE layers differentially expressed Nestin (Nes) in a manner suggesting that they respectively shared origins with neural retina (NR) and pigmented epithelial (RPE) layers. Transgenic marking enabled the enrichment of Nes-expressing IPE cells ex vivo, revealing a pronounced capacity to form neurospheres and differentiate into photoreceptor cells. IPE cells that did not express Nes were less able to form neurospheres, but a subset initiated the expression of pan-neural markers in primary adherent culture. These data collectively suggest that discrete populations of highly-pigmented cells with heterogeneous developmental potencies exist postnatally within the IPE, and that some of them are able to differentiate into multiple neuronal cell types. (c) 2006 Elsevier Inc. All rights reserved.


Link information
DOI
https://doi.org/10.1016/j.ydbio.2006.12.047
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000245290600035&DestApp=WOS_CPL
URL
http://orcid.org/0000-0001-6146-5184
ID information
  • DOI : 10.1016/j.ydbio.2006.12.047
  • ISSN : 0012-1606
  • ORCID - Put Code : 26053920
  • Web of Science ID : WOS:000245290600035

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