Papers

Peer-reviewed Corresponding author
Jan, 2006

Retinal stem/progenitor properties of iris pigment epithelial cells

DEVELOPMENTAL BIOLOGY
  • GW Sun
  • ,
  • M Asami
  • ,
  • H Ohta
  • ,
  • J Kosaka
  • ,
  • M Kosaka

Volume
289
Number
1
First page
243
Last page
252
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.ydbio.2005.10.035
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE

Neural stem cells/progenitors that give rise to neurons and glia have been identified in different regions of the brain.. including the embryonic retina and ciliary epithelium of the adult eye. Here, we first demonstrate the characterization of neural stem/progenitors in postnatal iris pigment epithelial (IPE) cells. Pure isolated IPE cells could form spheres that contained cells expressing retinal progenitor markers in non-adherent culture. The spheres grew by cell proliferation, as indicated by bromodeoxyuridine incorporation. When attached to laminin, the spheres forming IPE derived cells were able to exhibit neural phenotypes, including retinal-specific neurons. When co-cultured with embryonic retinal cells, or grafted into embryonic retina in vivo, the IPE cells could also display the phenotypes of photoreceptor neurons and Muller glia. Our results suggest that the IPE derived cells have retinal stem/progenitor properties and neurogenic potential without gene transfer, thereby providing a novel potential source for both basic stem cell biology and therapeutic applications for retinal diseases. (c) 2005 Elsevier Inc. All rights reserved.


Link information
DOI
https://doi.org/10.1016/j.ydbio.2005.10.035
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000234456200021&DestApp=WOS_CPL
ID information
  • DOI : 10.1016/j.ydbio.2005.10.035
  • ISSN : 0012-1606
  • Web of Science ID : WOS:000234456200021

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