Papers

Peer-reviewed
Mar, 2004

Adult human retinal pigment epithelial cells capable of differentiating into neurons

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • K Amemiya
  • ,
  • M Haruta
  • ,
  • M Takahashi
  • ,
  • M Kosaka
  • ,
  • G Eguchi

Volume
316
Number
1
First page
1
Last page
5
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.bbrc.2004.01.172
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE

We investigated the ability of adult human RPE cells to differentiate into neurons. Two cell lines were evaluated. The cells were cultured in medium with 8% serum, transferred to a neural stem cell maintenance culture, and induced to differentiate with retinoic acid. The cells were immunocytochemically examined at each step. The cells showed epithelial-like morphology with 8% serum and all were immunoreactive for beta-III tubulin. After transfer to the stem cell maintenance culture, they changed morphologically and became immunoreactive for MAP5 and neurofilament200 after inducement with retinoic acid. The ratio of MAP5 positive cells was higher in the young adult RPE cell line. No GFAP or rod-opsin immunoreactive cells were observed. Adult human RPE cells even from old person are capable of differentiating into neurons, although the ratio of mature neurons was greater in the young than in the old cell line in this condition. (C) 2004 Elsevier Inc. All rights reserved.


Link information
DOI
https://doi.org/10.1016/j.bbrc.2004.01.172
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220105800001&DestApp=WOS_CPL
ID information
  • DOI : 10.1016/j.bbrc.2004.01.172
  • ISSN : 0006-291X
  • Web of Science ID : WOS:000220105800001

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