Papers

Peer-reviewed Lead author Corresponding author
Dec, 1998

In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation

EXPERIMENTAL CELL RESEARCH
  • M Kosaka
  • ,
  • R Kodama
  • ,
  • G Eguchi

Volume
245
Number
2
First page
245
Last page
251
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1006/excr.1998.4211
Publisher
ACADEMIC PRESS INC

Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day-old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids, This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development, (C) 1998 Academic Press.


Link information
DOI
https://doi.org/10.1006/excr.1998.4211
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000077559800002&DestApp=WOS_CPL
ID information
  • DOI : 10.1006/excr.1998.4211
  • ISSN : 0014-4827
  • Web of Science ID : WOS:000077559800002

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