Dec, 1998
In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation
EXPERIMENTAL CELL RESEARCH
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- ,
- Volume
- 245
- Number
- 2
- First page
- 245
- Last page
- 251
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1006/excr.1998.4211
- Publisher
- ACADEMIC PRESS INC
Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day-old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids, This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development, (C) 1998 Academic Press.
- Link information
- ID information
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- DOI : 10.1006/excr.1998.4211
- ISSN : 0014-4827
- Web of Science ID : WOS:000077559800002