論文

査読有り 国際誌
2015年11月

Brain Rewarding Stimulation Reduces Extracellular Glutamate Through Glial Modulation in Medial Prefrontal Cortex of Rats.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
  • Gen Murakami
  • ,
  • Masato Nakamura
  • ,
  • Masatoshi Takita
  • ,
  • Yasushi Ishida
  • ,
  • Takatoshi Ueki
  • ,
  • Daiichiro Nakahara

40
12
開始ページ
2686
終了ページ
95
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/npp.2015.115
出版者・発行元
NATURE PUBLISHING GROUP

Growing evidence implicates a critical involvement of prefrontal glial modulation of extracellular glutamate (GLU) in aversive behaviors. However, nothing is known about whether prefrontal glial cells modulate GLU levels in rewarding behaviors. To address this question, we measured GLU efflux in the medial prefrontal cortex (PFC) of rats associated with rewarding behaviors. We used intracranial self-stimulation (ICSS) of the medial forebrain bundle (MFB) as the rewarding behavior. GLU was indirectly measured using microdialysis combined with on-line fluorometric detection of NADH resulting from the reaction of GLU and NAD(+) catalyzed by GLU dehydrogenase with a time resolution of 1 min. ICSS caused a minute-by-minute change of extracellular GLU in the medial PFC, with a slight decrease during the stimulation, followed by an increase afterward. This bidirectional change was tetrodotoxin insensitive and abolished by the gliotoxin fluorocitrate. To confirm and extend the previous studies of aversion-induced increase of extracellular GLU in the medial PFC, we also measured prefrontal GLU efflux associated with an aversive stimulation, immobilization stress. The temporal change in extracellular GLU caused by this stress was markedly different from that observed during ICSS. A rapid increase in GLU was detected during the aversive stimulation, followed by a large increase afterward. This bimodal change was tetrodotoxin insensitive, similar to that detected for ICSS. These findings indicate a bidirectional regulation of extracellular GLU by prefrontal glial cells associated with rat ICSS behavior, and reveal that glial modulation of GLU neurochemistry in the medial PFC contributes to rewarding as well as aversive behaviors in rats.

リンク情報
DOI
https://doi.org/10.1038/npp.2015.115
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25924203
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4864644
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000362836700004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/npp.2015.115
  • ISSN : 0893-133X
  • eISSN : 1740-634X
  • PubMed ID : 25924203
  • PubMed Central 記事ID : PMC4864644
  • Web of Science ID : WOS:000362836700004

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