2017年11月
CD34-negative hematopoietic stem cells show distinct expression profiles of homing molecules that limit engraftment in mice and sheep
INTERNATIONAL JOURNAL OF HEMATOLOGY
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- 巻
- 106
- 号
- 5
- 開始ページ
- 631
- 終了ページ
- 637
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s12185-017-2290-5
- 出版者・発行元
- SPRINGER JAPAN KK
We and others have reported that human hematopoietic stem cells (HSCs) are also present in the CD34-negative (CD34(-)) fraction of human cord blood (CB). Here, we examined the hematopoietic engraftment potential of 13 or 18 lineage-negative (13Lin(-) or 18Lin(-)) CD34(+/-) cells from human CB in mice and sheep. Both 13Lin(-) and 18Lin(-) CD34(+) cells efficiently engrafted in mice irrespective of transplantation route, be it by tail-vein injection (TVI) or by intra-bone marrow injection (IBMI). These cells also engrafted in sheep after in utero fetal intra-hepatic injection (IHI). In contrast, neither 13Lin(-) nor 18Lin(-) CD34(-) cells engrafted in either mice or sheep when transplanted by regular routes (i.e., TVI and fetal IHI, respectively), although both 13Lin(-) and 18Lin(-) CD34(-) cells engrafted in mice when transplanted by IBMI and exhibited multilineage reconstitution ability. Thus, the homing ability of CD34(-) HSCs is significantly more limited than that of CD34(+) HSCs. As for 18Lin(-), CD34(-) HSCs are characterized by low expression of the tetraspanin CD9, which promotes homing, and high expression of the peptidase CD26, which inhibits homing. This unique expression pattern homing-related molecules on CD34(-) HSCs could thus explain in part their reduced ability to home to the BM niche.
- リンク情報
- ID情報
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- DOI : 10.1007/s12185-017-2290-5
- ISSN : 0925-5710
- eISSN : 1865-3774
- PubMed ID : 28687990
- Web of Science ID : WOS:000414166500005