<p>Bone formation<italic>in vivo</italic>occurs in alkaline environment, which determines the optimal p<italic>K</italic>_aof phosphatases, the optimal amount of calcium for mineral precipitation, and the spherical shape of initial minerals. Manipulation of environmental pH for<italic>in vitro</italic>synthesis of bone-like tissue, showed a markedly rapid mineralization with nanofragments and alkaline milieu.</p>