2019年5月
Biomimetic mineralization using matrix vesicle nanofragments
Journal of Biomedical Materials Research Part A
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- 巻
- 107
- 号
- 5
- 開始ページ
- 1021
- 終了ページ
- 1030
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jbm.a.36618
- 出版者・発行元
- Wiley
In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. (c) 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021-1030, 2019.
- リンク情報
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- DOI
- https://doi.org/10.1002/jbm.a.36618
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30675987
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594056
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000462623800012&DestApp=WOS_CPL
- URL
- https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbm.a.36618
- URL
- https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jbm.a.36618
- ID情報
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- DOI : 10.1002/jbm.a.36618
- ISSN : 1549-3296
- eISSN : 1552-4965
- PubMed ID : 30675987
- PubMed Central 記事ID : PMC6594056
- Web of Science ID : WOS:000462623800012