論文

査読有り 国際誌
2019年5月15日

The very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function in fission yeast cells.

Journal of cell science
  • Yasuha Kinugasa
  • ,
  • Yasuhiro Hirano
  • ,
  • Megumi Sawai
  • ,
  • Yusuke Ohno
  • ,
  • Tomoko Shindo
  • ,
  • Haruhiko Asakawa
  • ,
  • Yuji Chikashige
  • ,
  • Shinsuke Shibata
  • ,
  • Akio Kihara
  • ,
  • Tokuko Haraguchi
  • ,
  • Yasushi Hiraoka

132
10
記述言語
英語
掲載種別
DOI
10.1242/jcs.229021

In eukaryotic cells, chromosomes are confined to the nucleus, which is compartmentalized by the nuclear membranes; these are continuous with the endoplasmic reticulum membranes. Maintaining the homeostasis of these membranes is an important cellular activity performed by lipid metabolic enzymes. However, how lipid metabolic enzymes affect nuclear membrane functions remains to be elucidated. We found that the very-long-chain fatty acid elongase Elo2 is located in the nuclear membrane and prevents lethal defects associated with nuclear membrane ruptures in mutants of the nuclear membrane proteins Lem2 and Bqt4 in the fission yeast Schizosaccharomyces pombe. Lipid composition analysis shows that t20:0/24:0 phytoceramide (a conjugate of C20:0 phytosphingosine and C24:0 fatty acid) is a major ceramide species in S. pombe The quantity of this ceramide is reduced in the absence of Lem2, and restored by increased expression of Elo2. Furthermore, loss of S. pombe Elo2 can be rescued by its human orthologs. These results suggest that the conserved very-long-chain fatty acid elongase producing the ceramide component is essential for nuclear membrane integrity and cell viability in eukaryotes.This article has an associated First Person interview with the first author of the paper.

リンク情報
DOI
https://doi.org/10.1242/jcs.229021
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30975915