2022年1月
Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
Communications Biology
- 巻
- 5
- 号
- 1
- 開始ページ
- 78
- 終了ページ
- 78
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s42003-022-03021-8
- 出版者・発行元
- Springer Science and Business Media LLC
<title>Abstract</title>DNA transfection is an important technology in life sciences, wherein nuclear entry of DNA is necessary to express exogenous DNA. Non-viral vectors and their transfection reagents are useful as safe transfection tools. However, they have no effect on the transfection of non-proliferating cells, the reason for which is not well understood. This study elucidates the mechanism through which transfected DNA enters the nucleus for gene expression. To monitor the behavior of transfected DNA, we introduce plasmid bearing <italic>lacO</italic> repeats and RFP-coding sequences into cells expressing GFP-LacI and observe plasmid behavior and RFP expression in living cells. RFP expression appears only after mitosis. Electron microscopy reveals that plasmids are wrapped with nuclear envelope (NE)‒like membranes or associated with chromosomes at telophase. The depletion of BAF, which is involved in NE reformation, delays plasmid RFP expression. These results suggest that transfected DNA is incorporated into the nucleus during NE reformation at telophase.
- リンク情報
- ID情報
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- DOI : 10.1038/s42003-022-03021-8
- eISSN : 2399-3642
- PubMed ID : 35058555
- PubMed Central 記事ID : PMC8776997