論文

査読有り 国際誌
2015年8月

Identification and purification of target protein using affinity resin bearing a photo-labile linker

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
  • Miyuki Mabuchi
  • ,
  • Tadashi Shimizu
  • ,
  • Masayuki Haramura
  • ,
  • Akito Tanaka

25
16
開始ページ
3373
終了ページ
3377
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bmcl.2015.05.023
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

This Letter presents an effective method for the identification of target proteins of bioactive compounds such as drugs, natural products, and intrinsic ligands, using an affinity resin. The application of a photo-labile linker to an affinity resin enabled the selective elution of a target protein by irradiation for a short duration at 4 degrees C while leaving a large amount of non-specific binding protein on the resin. We have named this method the 'STEAP' method (selective target elution from affinity resins with photo-labile linker). Only a target protein that can bind the bioactive compound, the so-called 'active' protein, is eluted by the selective cleavage of the linker between the solid matrix and the target compound, and therefore, it is worth considering the potential of this method for the hyper-purification of proteins. (C) 2015 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1016/j.bmcl.2015.05.023
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26099537
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000357572000060&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84934850141&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84934850141&origin=inward

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