論文

査読有り 国際誌
2011年9月

Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing

JOURNAL OF PROTEOMICS
  • Kohji Nagano
  • ,
  • Takashi Shinkawa
  • ,
  • Kuniyasu Kato
  • ,
  • Noriyuki Inomata
  • ,
  • Nami Yabuki
  • ,
  • Masayuki Haramura

74
10
開始ページ
1985
終了ページ
1993
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jprot.2011.05.019
出版者・発行元
ELSEVIER SCIENCE BV

We performed here MS-based cell surface proteome profiling of HCT-116 cells by two distinct methods based on biotin labeling and glycoprotein capturing. In total, 742 biotinylated and 219 glycosylated proteins were identified by the biotin labeling and glycoprotein capturing, of which 224 and 138 proteins known to be located on plasma membrane were included, respectively, according to ingenuity pathway analysis. Although 104 plasma membrane proteins were identified by both methods, the rest of 154 were identified only by one. Almost all the identified plasma membrane proteins possessed consensus N-glycosylation sites, and proteins having various numbers of glycosylation sites were identified by both methods. Thus, the discrepancies of the identified proteins obtained from those two methods might not be only due to the number of glycosylation sites, but also to the expression and/or glycosylation level of the cell surface proteins. We also identified 312 N-glycosylated proteins from xenograft samples by glycoprotein capturing of which 135 were known as plasma membrane proteins. Although a number of highly-expressed plasma membrane proteins were common between culture and xenograft cells, some proteins showed culture- or xenograft-specific expression, suggesting that those proteins might contribute to grow in different environment. (C) 2011 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 9

リンク情報
DOI
https://doi.org/10.1016/j.jprot.2011.05.019
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21621025
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000295302800015&DestApp=WOS_CPL
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80052023305&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=80052023305&origin=inward

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