論文

査読有り
2013年2月1日

Identification of ZNF395 as a novel modulator of adipogenesis

Experimental Cell Research
  • Ryota Hasegawa
  • ,
  • Yasuhiro Tomaru
  • ,
  • Michiel de Hoon
  • ,
  • Harukazu Suzuki
  • ,
  • Yoshihide Hayashizaki
  • ,
  • Jay W. Shin

319
3
開始ページ
68
終了ページ
76
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.yexcr.2012.11.003
出版者・発行元
Academic Press Inc.

Adipogenesis is the process of cell differentiation by which mesenchymal stem cells (MSC) become adipocytes. Investigating the transcriptional regulatory process during adipogenesis may provide strategies to prevent obesity and other metabolic disorders. In recent years, numerous zinc finger proteins (ZFPs) have been implicated in regulating differentiation and cell fate determination. To investigate the regulatory role of ZFPs involved in adipogenesis, we performed genome-wide microarray expression profiling of an adipogenesis time series. Particularly focusing on the transiently responsive ZFPs, we identified and characterized the functional role of ZNF395 in adipogenesis. A systematic ablation of the ZNF395 transcript during adipogenesis revealed 40% reduction of adipocytes when compared to control. Furthermore, the number of adipocytes as well as the expression of key adipocyte markers were greatly induced when MSC were co-transduced with ZNF395 and PPARG2. To further elucidate the functional role of ZNF395 during adipogenesis, we attempted to trans-differentiate human dermal fibroblasts with PPARG2. The test remarkably revealed that ZNF395 in conjunction with PPARG2 greatly induced adipogenesis from dermal fibroblasts when compared to PPARG2 alone. These loss and gain of function experiments firmly establish that ZNF395 coordinate the transcriptional regulatory pathway with PPARG2, which may be necessary for the genesis of adipocytes. © 2012 Elsevier Inc.

リンク情報
DOI
https://doi.org/10.1016/j.yexcr.2012.11.003
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23142027
ID情報
  • DOI : 10.1016/j.yexcr.2012.11.003
  • ISSN : 1090-2422
  • ISSN : 0014-4827
  • PubMed ID : 23142027
  • SCOPUS ID : 84872395865

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