Papers

Peer-reviewed
Oct, 2012

Sites of action of ghrelin receptor ligands in cardiovascular control

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
  • Brid Callaghan
  • Billie Hunne
  • Haruko Hirayama
  • Daniela M. Sartor
  • Trung V. Nguyen
  • Fe C. Abogadie
  • Dorota Ferens
  • Peter McIntyre
  • Kung Ban
  • Jonathan Baell
  • John B. Furness
  • James A. Brock
  • Display all

Volume
303
Number
8
First page
H1011
Last page
H1021
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1152/ajpheart.00418.2012
Publisher
AMER PHYSIOLOGICAL SOC

Callaghan B, Hunne B, Hirayama H, Sartor DM, Nguyen TV, Abogadie FC, Ferens D, McIntyre P, Ban K, Baell J, Furness JB, Brock JA. Sites of action of ghrelin receptor ligands in cardiovascular control. Am J Physiol Heart Circ Physiol 303: H1011-H1021, 2012. First published August 10, 2012; doi:10.1152/ajpheart.00418.2012.-Circulating ghrelin reduces blood pressure, but the mechanism for this action is unknown. This study investigated whether ghrelin has direct vasodilator effects mediated through the growth hormone secretagogue receptor 1a (GHSR1a) and whether ghrelin reduces sympathetic nerve activity. Mice expressing enhanced green fluorescent protein under control of the promoter for growth hormone secretagogue receptor (GHSR) and RT-PCR were used to locate sites of receptor expression. Effects of ghrelin and the nonpeptide GHSR1a agonist capromorelin on rat arteries and on transmission in sympathetic ganglia were measured in vitro. In addition, rat blood pressure and sympathetic nerve activity responses to ghrelin were determined in vivo. In reporter mice, expression of GHSR was revealed at sites where it has been previously demonstrated (hypothalamic neurons, renal tubules, sympathetic preganglionic neurons) but not in any artery studied, including mesenteric, cerebral, and coronary arteries. In rat, RT-PCR detected GHSR1a mRNA expression in spinal cord and kidney but not in the aorta or in mesenteric arteries. Moreover, the aorta and mesenteric arteries from rats were not dilated by ghrelin or capromorelin at concentrations >100 times their EC50 determined in cells transfected with human or rat GHSR1a. These agonists did not affect transmission from preganglionic sympathetic neurons that express GHSR1a. Intravenous application of ghrelin lowered blood pressure and decreased splanchnic nerve activity. It is concluded that the blood pressure reduction to ghrelin occurs concomitantly with a decrease in sympathetic nerve activity and is not caused by direct actions on blood vessels or by inhibition of transmission in sympathetic ganglia.

Link information
DOI
https://doi.org/10.1152/ajpheart.00418.2012
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22886413
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000309981600008&DestApp=WOS_CPL
ID information
  • DOI : 10.1152/ajpheart.00418.2012
  • ISSN : 0363-6135
  • Pubmed ID : 22886413
  • Web of Science ID : WOS:000309981600008

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