Papers

Peer-reviewed International journal
Nov 30, 2004

Invasion of chicken embryo fibroblast cells by avian Pasteurella multocida.

Veterinary microbiology
  • Hussam Al-haj Ali
  • ,
  • Takuo Sawada
  • ,
  • Hitoshi Hatakeyama
  • ,
  • Yoshiya Katayama
  • ,
  • Noriyuki Ohtsuki
  • ,
  • Osamu Itoh

Volume
104
Number
1-2
First page
55
Last page
62
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.vetmic.2004.08.008
Publisher
ELSEVIER SCIENCE BV

Invasion of chicken embryo fibroblast (CEF) cells by the virulent encapsulated Pasteurella multocida strains P-1059 (serovar A:3) and X-73 (serovar A:1) and an avirulent noncapsulated derivative P-1059B (serovar -:3) was investigated. The number of intracellular bacteria increased for all the strains after 2, 4 and 6 h post-inoculation to CEF cells. By 6 h post-inoculation, the number of invaded bacteria of encapsulated strains was significantly higher than noncapsulated strain and reached 150- and 112-fold for strains P-1059 and X-73, respectively, while it was 9-fold for strain P-1059B as compared to the number of invaded bacteria recovered after 2 h post-inoculation. Electron microscopy of invasion by encapsulated strains showed that the bacteria were adhering to CEF cells membrane after 1 h of inoculation. By 4-h, one or two bacteria were detected within membrane-bound vacuoles of the intracellular space. The number of intracellular bacteria markedly increased at 14 h post-inoculation. Invasion of all strains was inhibited significantly when the monolayers were treated with periodic acid (P<0.001) or trypsin (P<0.05). The treatment of bacteria with hyaluronidase did not affect invasion. The present results indicate that avian P. multocida capsular type A strains are invasive and that the receptor on CEF cell surface might be glycoprotein.

Link information
DOI
https://doi.org/10.1016/j.vetmic.2004.08.008
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15530739
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000225329000005&DestApp=WOS_CPL
ID information
  • DOI : 10.1016/j.vetmic.2004.08.008
  • ISSN : 0378-1135
  • Pubmed ID : 15530739
  • Web of Science ID : WOS:000225329000005

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