Papers

International journal
Oct 18, 2022

Phosphorylation of Tau at Threonine 231 in Patients With Multiple System Atrophy and in a Mouse Model.

Journal of neuropathology and experimental neurology
  • Makoto T Tanaka
  • ,
  • Kunikazu Tanji
  • ,
  • Yasuo Miki
  • ,
  • Taku Ozaki
  • ,
  • Fumiaki Mori
  • ,
  • Hideki Hayashi
  • ,
  • Akiyoshi Kakita
  • ,
  • Koichi Wakabayashi

Volume
81
Number
11
First page
920
Last page
930
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1093/jnen/nlac082

Multiple system atrophy (MSA) is a sporadic neurodegenerative disorder pathologically characterized by the presence of glial cytoplasmic inclusions (GCIs). Some MSA patients exhibit motor deficits with accompanying cognitive impairment. Of note, some patients suffering from MSA with longer disease duration have AT8-positive signals, which correspond to phosphorylated tau (P-tau) at 202/205 (P-tau202/205). However, P-tau sites other than the AT8 antibody epitope antibody are less well studied. Here, we focused on the effect of α-synuclein (Syn) expression on the phosphorylation of tau in MSA model mice. Among the 6 kinds of antibodies against P-tau, we confirmed that antibodies against P-tau at 231 (P-tau231) were phospho-specific and found that P-tau231 level was increased in parallel with disease progression in MSA model mice. Additional studies of human brains revealed that P-tau231 was mainly expressed in the temporal cortex in MSA brains and that its expression level was significantly higher in MSA patients than in controls. Immunohistochemical analysis showed that anti-P-tau231-, but not AT8, antibodies mainly immunolabeled hippocampal CA2/3 pyramidal neurons, and some GCIs in MSA. These data suggest that P-tau231 occurs in MSA differently from P-tau202/205.

Link information
DOI
https://doi.org/10.1093/jnen/nlac082
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/36083205
ID information
  • DOI : 10.1093/jnen/nlac082
  • Pubmed ID : 36083205

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