論文

国際誌
2021年8月23日

Chromosome-level genome assembly of japanese chestnut (Castanea crenata Sieb. Et Zucc.) reveals conserved chromosomal segments in woody rosids.

DNA research : an international journal for rapid publication of reports on genes and genomes
  • Kenta Shirasawa
  • ,
  • Sogo Nishio
  • ,
  • Shingo Terakami
  • ,
  • Roberto Botta
  • ,
  • Daniela Torello Marinoni
  • ,
  • Sachiko Isobe

28
5
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/dnares/dsab016

Japanese chestnut (Castanea crenata Sieb. et Zucc.), unlike other Castanea species, is resistant to most diseases and wasps. However, genomic data of Japanese chestnut that could be used to determine its biotic stress resistance mechanisms have not been reported to date. In this study, we employed long-read sequencing and genetic mapping to generate genome sequences of Japanese chestnut at the chromosome level. Long reads (47.7 Gb; 71.6× genome coverage) were assembled into 781 contigs, with a total length of 721.2 Mb and a contig N50 length of 1.6 Mb. Genome sequences were anchored to the chestnut genetic map, comprising 14,973 single nucleotide polymorphisms (SNPs) and covering 1,807.8 cM map distance, to establish a chromosome-level genome assembly (683.8 Mb), with 69,980 potential protein-encoding genes and 425.5 Mb repetitive sequences. Furthermore, comparative genome structure analysis revealed that Japanese chestnut shares conserved chromosomal segments with woody plants, but not with herbaceous plants, of rosids. Overall, the genome sequence data of Japanese chestnut generated in this study is expected to enhance not only its genetics and genomics but also the evolutionary genomics of woody rosids.

リンク情報
DOI
https://doi.org/10.1093/dnares/dsab016
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34424280
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8435554
ID情報
  • DOI : 10.1093/dnares/dsab016
  • PubMed ID : 34424280
  • PubMed Central 記事ID : PMC8435554

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