論文

国際誌
2021年9月23日

Role of pyruvate in maintaining cell viability and energy production under high-glucose conditions.

Scientific reports
  • Hideji Yako
  • ,
  • Naoko Niimi
  • ,
  • Ayako Kato
  • ,
  • Shizuka Takaku
  • ,
  • Yasuaki Tatsumi
  • ,
  • Yasumasa Nishito
  • ,
  • Koichi Kato
  • ,
  • Kazunori Sango

11
1
開始ページ
18910
終了ページ
18910
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-021-98082-w

Pyruvate functions as a key molecule in energy production and as an antioxidant. The efficacy of pyruvate supplementation in diabetic retinopathy and nephropathy has been shown in animal models; however, its significance in the functional maintenance of neurons and Schwann cells under diabetic conditions remains unknown. We observed rapid and extensive cell death under high-glucose (> 10 mM) and pyruvate-starved conditions. Exposure of Schwann cells to these conditions led to a significant decrease in glycolytic flux, mitochondrial respiration and ATP production, accompanied by enhanced collateral glycolysis pathways (e.g., polyol pathway). Cell death could be prevented by supplementation with 2-oxoglutarate (a TCA cycle intermediate), benfotiamine (the vitamin B1 derivative that suppresses the collateral pathways), or the poly (ADP-ribose) polymerase (PARP) inhibitor, rucaparib. Our findings suggest that exogenous pyruvate plays a pivotal role in maintaining glycolysis-TCA cycle flux and ATP production under high-glucose conditions by suppressing PARP activity.

リンク情報
DOI
https://doi.org/10.1038/s41598-021-98082-w
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34556698
ID情報
  • DOI : 10.1038/s41598-021-98082-w
  • PubMed ID : 34556698

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