論文

2023年3月

Ex vivo chemosensitivity assay using primary ovarian cancer organoids for predicting clinical response and screening effective drugs

Human Cell
  • Yu Ito
  • Jumpei Kondo
  • Masamune Masuda
  • Shinya Matsuzaki
  • Kunishige Onuma
  • Mizuki Kanda
  • Yuko Watanabe
  • Hitomi Sakaguchi
  • Kiyoshi Yoshino
  • Yutaka Ueda
  • Shoji Kamiura
  • Tadashi Kimura
  • Masahiro Inoue
  • 全て表示

36
2
開始ページ
752
終了ページ
761
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s13577-022-00827-w

Selecting the best treatment for individual patients with cancer has attracted attention for improving clinical outcomes. Recent progress in organoid culture may lead to the development of personalized medicine. Unlike molecular-targeting drugs, there are no predictive methods for patient response to standard chemotherapies for ovarian cancer. We prepared organoids using the cancer tissue-originated spheroid (CTOS) method from 61 patients with ovarian cancer with 100% success rate. Chemosensitivity assays for paclitaxel and carboplatin were performed with 84% success rate using the primary organoids from 50 patients who received the chemotherapy. A wide range of sensitivities was observed among organoids for both drugs. All four clinically resistant organoids were resistant to both drugs in 18 cases in which clinical response information was available. Five out of 18 cases (28%) were double-resistant, the response rate of which was compatible with the clinical remission rate. Carboplatin was significantly more sensitive in serous than in clear cell subtypes (P = 0.025). We generated two lines of organoids, screened 1135 drugs, and found several drugs with better combinatory effects with carboplatin than with paclitaxel. Some drugs, including afatinib, have shown an additive effect with carboplatin. The organoid sensitivity assay did not predict the clinical outcomes, both progression free and overall survival.

リンク情報
DOI
https://doi.org/10.1007/s13577-022-00827-w
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/36474106
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85143442809&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85143442809&origin=inward
ID情報
  • DOI : 10.1007/s13577-022-00827-w
  • ISSN : 0914-7470
  • eISSN : 1749-0774
  • PubMed ID : 36474106
  • SCOPUS ID : 85143442809

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