2016年7月
Peptidoglycan Microarray as a Novel Tool to Explore Protein-Ligand Recognition
BIOPOLYMERS
- ,
- ,
- ,
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- 巻
- 106
- 号
- 4
- 開始ページ
- 422
- 終了ページ
- 429
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/bip.22807
- 出版者・発行元
- WILEY-BLACKWELL
Peptidoglycan is a giant bag-shaped molecule essential for bacterial cell shape and resistance to osmotic stresses. The activity of a large number of bacterial surface proteins involved in cell growth and division requires binding to this macromolecule. Recognition of peptidoglycan by immune effectors is also crucial for the establishment of the immune response against pathogens. The availability of pure and chemically defined peptidoglycan fragments is a major technical bottleneck that has precluded systematic studies of the mechanisms underpinning protein-mediated peptidoglycan recognition. Here, we report a microarray strategy suitable to carry out comprehensive studies to characterize proteins-peptidoglycan interactions. We describe a method to introduce a functional group on peptidoglycan fragments allowing their stable immobilization on amorphous carbon chip plates to minimize nonspecific binding. Such peptidoglycan microarrays were used with a model peptidoglycan binding protein-the human peptidoglycan recognition protein-S (hPGRP-S). We propose that this strategy could be implemented to carry out high-throughput analyses to study peptidoglycan binding proteins. (C) 2016 Wiley Periodicals, Inc.
- リンク情報
- ID情報
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- DOI : 10.1002/bip.22807
- ISSN : 0006-3525
- eISSN : 1097-0282
- Web of Science ID : WOS:000393460600005