2014年12月
In Vivo Role of Phosphorylation of Cryptochrome 2 in the Mouse Circadian Clock
MOLECULAR AND CELLULAR BIOLOGY
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 34
- 号
- 24
- 開始ページ
- 4464
- 終了ページ
- 4473
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1128/MCB.00711-14
- 出版者・発行元
- AMER SOC MICROBIOLOGY
The circadian clock is finely regulated by posttranslational modifications of clock components. Mouse CRY2, a critical player in the mammalian clock, is phosphorylated at Ser557 for proteasome-mediated degradation, but its in vivo role in circadian organization was not revealed. Here, we generated CRY2(S557A) mutant mice, in which Ser557 phosphorylation is specifically abolished. The mutation lengthened free-running periods of the behavioral rhythms and PER2:: LUC bioluminescence rhythms of cultured liver. In livers from mutant mice, the nuclear CRY2 level was elevated, with enhanced PER2 nuclear occupancy and suppression of E-box-regulated genes. Thus, Ser557 phosphorylation-dependent regulation of CRY2 is essential for proper clock oscillation in vivo.
- リンク情報
- ID情報
-
- DOI : 10.1128/MCB.00711-14
- ISSN : 0270-7306
- eISSN : 1098-5549
- PubMed ID : 25288642
- Web of Science ID : WOS:000345223000008