平田真樹 (Maki Hirata) - 論文

Disruption of cell proliferation and apoptosis balance in the testes of crossbred cattle‐yaks affects spermatogenic cell fate and sterility

Megumi Shimazaki, Manita Wittayarat, Rentsenkhand Sambuu, Asami Sugita, Masaki Kawaguchi, Maki Hirata, Fuminori Tanihara, Mitsuhiro Takagi, Masayasu Taniguchi, Takeshige Otoi, Yoko Sato

Reproduction in Domestic Animals 2022年6月6日

Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system.

Zhao Namula, Manita Wittayarat, Lanh Thi Kim Do, Thanh Van Nguyen, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Takeshige Otoi

Veterinary and animal science 16 100241-100241 2022年6月

Viability and developmental potential of porcine blastocysts preserved for short term in a chemically defined medium at ambient temperature.

Qingyi Lin, Quynh Anh Le, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Chommanart Thongkittidilok, Osamu Sawamoto, Takeshi Kikuchi, Takeshige Otoi

Reproduction in domestic animals = Zuchthygiene 57(5) 556-563 2022年5月

Aberrant levels of DNA methylation and H3K9 acetylation in the testicular cells of crossbred cattle-yak showing infertility.

Praopilas Phakdeedindan, Manita Wittayarat, Theerawat Tharasanit, Mongkol Techakumphu, Megumi Shimazaki, Rentsenkhand Sambuu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Takeshige Otoi, Yoko Sato

Reproduction in domestic animals = Zuchthygiene 57(3) 304-313 2022年3月

Effects of individual or in-combination antioxidant supplementation during in vitro maturation culture on the developmental competence and quality of porcine embryos.

Chommanart Thongkittidilok, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Thi Kim Lanh Do, Zhao Namula, Maki Hirata, Fuminori Tanihara, Takeshige Otoi

Reproduction in domestic animals = Zuchthygiene 57(3) 314-320 2022年3月

Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection.

Zhao Namula, Quynh Anh Le, Manita Wittayarat, Qingyi Lin, Koki Takebayashi, Maki Hirata, Lanh Thi Kim Do, Fuminori Tanihara, Takeshige Otoi

Veterinary world 15(2) 496-501 2022年2月

Zona pellucida treatment before CRISPR/Cas9-mediated genome editing of porcine zygotes.

Zhao Namula, Maki Hirata, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi

Veterinary medicine and science 8(1) 164-169 2022年1月

Short-term preservation of porcine zygotes at ambient temperature using a chemically defined medium.

Qingyi Lin, Quynh Anh Le, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Osamu Sawamoto, Takeshi Kikuchi, Takeshige Otoi

Animal science journal = Nihon chikusan Gakkaiho 93(1) e13711 2022年1月

Generation of mutant pigs by lipofection-mediated genome editing in embryos.

Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Taro Mito, Sayuri Tomonari, Fuminori Tanihara, Takeshige Otoi

Scientific reports 11(1) 23806-23806 2021年12月13日

Development and characterization of Gal KO porcine bone marrow-derived mesenchymal stem cells.

Takeshi Kikuchi, Masuhiro Nishimura, Maki Hirata, Fuminori Tanihara, Natsuki Komori, Makoto Tanaka, Osamu Sawamoto, Takeshige Otoi, Shinichi Matsumoto

Xenotransplantation 28(6) e12717 2021年11月

Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events.

Qingyi Lin, Quynh Anh Le, Koki Takebayashi, Chommanart Thongkittidilok, Manita Wittayarat, Maki Hirata, Fuminori Tanihara, Takeshige Otoi

BMC research notes 14(1) 389-389 2021年10月9日

Current status of the application of gene editing in pigs.

Fuminori Tanihara, Maki Hirata, Takeshige Otoi

The Journal of reproduction and development 67(3) 177-187 2021年6月21日

Vaginal stimulation enhances ovulation of queen ovaries treated using a combination of eCG and hCG

Naoaki Yoshimura, Masayasu Taniguchi, Tsukasa Terazono, Tetsushi Ono, Mitsuhiro Takagi, Yoko Sato, Maki Hirata, Fuminori Tanihara, Takeshige Otoi

Veterinary Medicine and Science 7(5) 1569-1574 2021年6月16日

One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis.

Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Takeshige Otoi

International journal of molecular sciences 22(5) 2021年2月24日

Lipofection-Mediated Introduction of CRISPR/Cas9 System into Porcine Oocytes and Embryos.

Maki Hirata, Manita Wittayarat, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Chommanart Thongkittidilok, Fuminori Tanihara, Takeshige Otoi

Animals : an open access journal from MDPI 11(2) 2021年2月23日

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages.

Quynh Anh Le, Fuminori Tanihara, Manita Wittayarat, Zhao Namula, Yoko Sato, Qingyi Lin, Koki Takebayashi, Maki Hirata, Takeshige Otoi

BMC research notes 14(1) 7-7 2021年1月6日

Improvement of the in vitro fertilization and embryo development using frozen-thawed spermatozoa of microminipigs.

Zhao Namula, Yasuhiro Isumi, Yoko Sato, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi

Archives animal breeding 64(1) 265-271 2021年

Introduction of a point mutation in the KRAS gene of in vitro fertilized porcine zygotes via electroporation of the CRISPR/Cas9 system with single-stranded oligodeoxynucleotides.

Manita Wittayarat, Maki Hirata, Zhao Namula, Yoko Sato, Nhien T Nguyen, Quynh A Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara, Takeshige Otoi

Animal science journal = Nihon chikusan Gakkaiho 92(1) e13534 2021年1月

Curcumin supplementation in the maturation medium improves the maturation, fertilisation and developmental competence of porcine oocytes.

Zhao Namula, Yoko Sato, Manita Wittayarat, Quynh Anh Le, Nhien Thi Nguyen, Qingyi Lin, Maki Hirata, Fuminori Tanihara, Takeshige Otoi

Acta veterinaria Hungarica 2020年11月20日

One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs.

Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Yoko Sato, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Takeshige Otoi

In vitro cellular & developmental biology. Animal 56(8) 614-621 2020年9月