論文

査読有り
2009年10月

Characterization of a Signaling Complex Composed of Sensory Rhodopsin I and Its Cognate Transducer Protein from the Eubacterium Salinibacter ruber

BIOCHEMISTRY
  • Yuki Sudo
  • ,
  • Akiko Okada
  • ,
  • Daisuke Suzuki
  • ,
  • Keiichi Inoue
  • ,
  • Hiroki Irieda
  • ,
  • Makoto Sakai
  • ,
  • Masaaki Fujii
  • ,
  • Yuji Furutani
  • ,
  • Hideki Kandori
  • ,
  • Michio Homma

48
42
開始ページ
10136
終了ページ
10145
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/bi901338d
出版者・発行元
AMER CHEMICAL SOC

Sensory rhodopsin I (SRI) exists in the cell membranes of microorganisms such as the archaeon Halobacterium salinarum and is a photosensor responsible for positive and negative phototaxis. SRI forms a signaling complex with its cognate transducer protei HtrI, in the membrane. That complex transmits light signals to the flagellar motor through changes in protein-protein interactions with the kinase CheA and the adaptor protein CheW, which controls the direction of the rotation of the flagellar motor. Recently, we cloned and characterized Salinibacter sensory rhodopsin I (SrSRI), which is the first SRI-like protein identified in eubacteria [Kitajima-Ihara, T., et al. (2008) J. Biol. Chem. 283, 23533-23541]. Here we cloned and expressed SrSRI with its full-length transducer protein, SrHtrI, as a fusion construct. We succeeded in producing the complex in Escherichia coli as a recombinant protein with high quality having all-trans-retinal as a chromophore for SRI, although the expression level was low (0.10 mg/L of culture). In addition, we report here the photochemical properties of the SrSRI-SrHtrI complex using time-resolved laser flash spectroscopy and other spectroscopic techniques and compare them to SrSRI without SrHtrI.

リンク情報
DOI
https://doi.org/10.1021/bi901338d
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19778064
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000270810000022&DestApp=WOS_CPL
ID情報
  • DOI : 10.1021/bi901338d
  • ISSN : 0006-2960
  • PubMed ID : 19778064
  • Web of Science ID : WOS:000270810000022

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