2021年9月
A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E
FEBS LETTERS
- ,
- ,
- ,
- ,
- 巻
- 595
- 号
- 18
- 開始ページ
- 2341
- 終了ページ
- 2349
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/1873-3468.14173
- 出版者・発行元
- WILEY
Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS-CoV-2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high-sensitivity screening of the GAG-binding specificity of proteins and applied it for the analysis of SARS-CoV-2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration-dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS-CoV-2 infection.
- リンク情報
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- DOI
- https://doi.org/10.1002/1873-3468.14173
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/34375459
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8427098
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000685525100001&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1002/1873-3468.14173
- ISSN : 0014-5793
- eISSN : 1873-3468
- PubMed ID : 34375459
- PubMed Central 記事ID : PMC8427098
- Web of Science ID : WOS:000685525100001